Synthesis of γ-N-modified 8-oxo-2’-deoxyguanosine triphosphate and its characterization

Ninticha Thavoncharoensub, Kento Maruyama, Choon Han Heh, Kok Hoong Leong, Hui Shi, Yoshiharu Shigematsu, Shigeki Sasaki, Yosuke Taniguchi

Research output: Contribution to journalArticle

Abstract

8-OxodGTP is generated by the reaction between dGTP and reactive oxygen species and a considered mutagenic nucleotide. It can be incorporated into the duplex DNA during replication processes by the DNA polymerase, and thus the repair enzyme removes oxodGTP from the nucleotide pools in living cells. On the other hand, the γ-modified triphosphates show interesting properties for use as biological tools. Therefore, the γ-N-pyrenylalkyl-oxodGTP derivatives were synthesized and their effect on the enzymatic reactions were evaluated. The γ-N-pyrenylmethyl-oxodGTP was found to be accepted by the DNA polymerase just like oxodGTP, but showed a competitive inhibition property for the human oxodGTPase.

Original languageEnglish
Pages (from-to)578-589
Number of pages12
JournalNucleosides, Nucleotides and Nucleic Acids
Volume38
Issue number8
DOIs
Publication statusPublished - Aug 3 2019

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Genetics

Cite this