Synthesis of DNA-peptide conjugates by solid-phase fragment condensation

Takanori Kubo, Masaaki Morikawa, Hideki Ohba, Masayuki Fujii

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

(Matrix presented) DNA-peptide conjugates were synthesized by condensing partially protected peptide fragments and oligonucleotides on a CPG support using diisocyanatoalkane as a linker. After cleavage and deprotection with aqueous ammonia, pure products were obtained by single RPHPLC purification in satisfactory yields and identified by a MALDI-TOF MS spectrometer. This method allows one to prepare DNA-peptide conjugates with any components and sequences of DNA and peptides.

Original languageEnglish
Pages (from-to)2623-2626
Number of pages4
JournalOrganic Letters
Volume5
Issue number15
DOIs
Publication statusPublished - Jul 24 2003
Externally publishedYes

Fingerprint

peptides
solid phases
Condensation
deoxyribonucleic acid
condensation
fragments
Peptides
DNA
synthesis
Peptide Fragments
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Ammonia
Oligonucleotides
Purification
condensing
Spectrometers
oligonucleotides
purification
ammonia
cleavage

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Synthesis of DNA-peptide conjugates by solid-phase fragment condensation. / Kubo, Takanori; Morikawa, Masaaki; Ohba, Hideki; Fujii, Masayuki.

In: Organic Letters, Vol. 5, No. 15, 24.07.2003, p. 2623-2626.

Research output: Contribution to journalArticle

Kubo, Takanori ; Morikawa, Masaaki ; Ohba, Hideki ; Fujii, Masayuki. / Synthesis of DNA-peptide conjugates by solid-phase fragment condensation. In: Organic Letters. 2003 ; Vol. 5, No. 15. pp. 2623-2626.
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