Synthetic gene circuit-mediated monitoring of endogenous metabolites: Identification of GAL11 as a novel multicopy enhancer of S-adenosylmethionine level in yeast

Taichi Umeyama, Satoshi Okada, Takashi Ito

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Monitoring levels of key metabolites in living cells comprises a critical step in various investigations. The simplest approach to this goal is a fluorescent reporter gene using an endogenous promoter responsive to the metabolite. However, such a promoter is often not identified or even present in the species of interest. An alternative can be a synthetic gene circuit based on a heterologous pair consisting of a promoter and a transcription factor known to respond to the metabolite. We exploited the met operator and MetJ repressor of Escherichia coli, the interaction between which depends on S-adenosylmethionine (SAM), to construct synthetic gene circuits that report SAM levels in Saccharomyces cerevisiae. Using a dual-input circuit that outputs selection marker genes in a doxycycline-tunable manner, we screened a genomic library to identify GAL11 as a novel multicopy enhancer of SAM levels. These results demonstrate the potential and utility of synthetic gene circuit-mediated metabolite monitoring.

Original languageEnglish
Pages (from-to)425-430
Number of pages6
JournalACS Synthetic Biology
Volume2
Issue number8
DOIs
Publication statusPublished - Aug 16 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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