Synthetic inositol 1,3,4,5-tetrakisphosphate analogues

Masato Hirata, Y. Kimura, T. Ishimatsu, Fumi Takahashi, T. Shuto, Toshiyuki Sasaguri, T. Koga, Y. Watanabe, S. Ozaki

Research output: Contribution to journalArticle

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Abstract

Inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4] analogues were synthesized and their effects on [3H]Ins(1,3,4,5)P4 5-phosphatase, [3H]Ins(1,3,4,5)P4 3-phosphatase and [3H]inositol 1,4,5-trisphosphate [3H]Ins(1,4,5)P3] 5-phosphatase activities were examined. The Ins(1,3,4,5)P4 analogue with the aminobenzoyl group at the 2-position of Ins(1,3,4,5)P4 inhibited the hydrolysis of 5-phosphate of [3H]Ins(1,3,4,5)P4 catalysed by erythrocyte ghosts, with a lower K(i) value than seen with Ins(1,3,4,5)P4, whereas the analogue with the aminocyclohexanecarbonyl group at the same position had a higher K(i) value. The Ins(1,4,5)P3 analogues that we had previously synthesized were also capable of inhibiting this process, with the same tendency as Ins(1,3,4,5)P4 analogues. Such differences in the potency among Ins(1,3,4,5)P4 and Ins(1,4,5)P3 analogues were applicable to other phosphatase activities, namely [3H]Ins(1,3,4,5)P4 3-phosphatase and [3H]Ins(1,4,5)P3 5-phosphatase. These results suggest that the active sites of these enzymes may catalyse the dephosphorylation in a similar fashion.

Original languageEnglish
Pages (from-to)333-336
Number of pages4
JournalBiochemical Journal
Volume276
Issue number2
DOIs
Publication statusPublished - Jan 1 1991

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Phosphoric Monoester Hydrolases
Inositol 1,4,5-Trisphosphate
inositol-1,3,4,5-tetrakisphosphate
Erythrocyte Membrane
Hydrolysis
Catalytic Domain
Phosphates
Enzymes
multiple inositol-polyphosphate phosphatase
Inositol Polyphosphate 5-Phosphatases

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hirata, M., Kimura, Y., Ishimatsu, T., Takahashi, F., Shuto, T., Sasaguri, T., ... Ozaki, S. (1991). Synthetic inositol 1,3,4,5-tetrakisphosphate analogues. Biochemical Journal, 276(2), 333-336. https://doi.org/10.1042/bj2760333

Synthetic inositol 1,3,4,5-tetrakisphosphate analogues. / Hirata, Masato; Kimura, Y.; Ishimatsu, T.; Takahashi, Fumi; Shuto, T.; Sasaguri, Toshiyuki; Koga, T.; Watanabe, Y.; Ozaki, S.

In: Biochemical Journal, Vol. 276, No. 2, 01.01.1991, p. 333-336.

Research output: Contribution to journalArticle

Hirata, M, Kimura, Y, Ishimatsu, T, Takahashi, F, Shuto, T, Sasaguri, T, Koga, T, Watanabe, Y & Ozaki, S 1991, 'Synthetic inositol 1,3,4,5-tetrakisphosphate analogues', Biochemical Journal, vol. 276, no. 2, pp. 333-336. https://doi.org/10.1042/bj2760333
Hirata M, Kimura Y, Ishimatsu T, Takahashi F, Shuto T, Sasaguri T et al. Synthetic inositol 1,3,4,5-tetrakisphosphate analogues. Biochemical Journal. 1991 Jan 1;276(2):333-336. https://doi.org/10.1042/bj2760333
Hirata, Masato ; Kimura, Y. ; Ishimatsu, T. ; Takahashi, Fumi ; Shuto, T. ; Sasaguri, Toshiyuki ; Koga, T. ; Watanabe, Y. ; Ozaki, S. / Synthetic inositol 1,3,4,5-tetrakisphosphate analogues. In: Biochemical Journal. 1991 ; Vol. 276, No. 2. pp. 333-336.
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