TY - JOUR
T1 - Synthetic ligand promotes gene expression by affecting GC sequence in promoter
AU - Matsumoto, Saki
AU - Iida, Kei
AU - Murata, Asako
AU - Denawa, Masatsugu
AU - Hagiwara, Masatoshi
AU - Nakatani, Kazuhiko
N1 - Funding Information:
The authors thank Dr. Masayasu Toyomoto of Kyoto University for technical assistance with the extraction of RNA. This study was supported by JSPS KAKENHI Grant-in-Aid for Specially Promoted Research (26000007) to KN, JSPS KAKENHI Grant-in-Aid for Scientific Research (C) (15K01820) to AM, Grants-in-Aid from the Japan Agency for Medical Research and Development (AMED) to MH and partially supported by KAKENHI Grant-in-Aid for JSPS Fellows (265460) to SM.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017
Y1 - 2017
N2 - A naphthyridine carbamate tetramer (NCT8) is a synthetic compound, which selectively binds to nucleic acids containing CGG/CGG sequence. Although NCT8 is a promising compound for a wide range of DNA and RNA based biotechnology such as modulation of specific gene expression, little is known about its behavior in human cells. In the present study, we investigated the changes induced in gene expression by NCT8. Genes differentially expressed in the presence of NCT8 in HeLa cells were identified by whole-transcriptome analysis. The whole-transcriptome analysis showed that NCT8 significantly induced up-regulation of specific genes, whose promoter region has GC-rich sequence.
AB - A naphthyridine carbamate tetramer (NCT8) is a synthetic compound, which selectively binds to nucleic acids containing CGG/CGG sequence. Although NCT8 is a promising compound for a wide range of DNA and RNA based biotechnology such as modulation of specific gene expression, little is known about its behavior in human cells. In the present study, we investigated the changes induced in gene expression by NCT8. Genes differentially expressed in the presence of NCT8 in HeLa cells were identified by whole-transcriptome analysis. The whole-transcriptome analysis showed that NCT8 significantly induced up-regulation of specific genes, whose promoter region has GC-rich sequence.
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U2 - 10.1016/j.bmcl.2017.06.006
DO - 10.1016/j.bmcl.2017.06.006
M3 - Article
C2 - 28610980
AN - SCOPUS:85020480809
VL - 27
SP - 3391
EP - 3394
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
SN - 0960-894X
IS - 15
ER -