Synthetic octacalcium phosphate-enhanced reparative dentine formation via induction of odontoblast differentiation

Xiaogu Wang, Tetsuo Suzawa, Tomohiko Miyauchi, Baohong Zhao, Rika Yasuhara, Takahisa Anada, Masanori Nakamura, Osamu Suzuki, Ryutaro Kamijo

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Synthetic octacalcium phosphate (OCP) has been suggested to be a useful biomaterial for the regeneration of hard tissues, including bone. However, it remains unknown whether OCP induces dentine formation by dental pulp. We investigated biomineralization of dental pulp exposed to synthetic OCP in vitro and in vivo. When dental pulp was exposed directly to OCP, rapid formation of reparative dentine (RD) was induced and expression of dentine sialoprotein synthesis was observed in dental pulp adjacent to newly synthesized RD. OCP inhibited the proliferation of rat pulp cells and also promoted their odontoblastic differentiation in vitro, as alkaline phosphatase activity, mineralization of pulp cells and the expression level of dentine sialophosphoprotein were enhanced. Direct contact between OCP and pulp cells is required for OCP to exhibit its effects in vitro. The expression level of Runx2, a transcription factor whose downregulation is closely related to odontoblast differentiation, was downregulated in pulp cells cultured with OCP. Structural changes of OCP during culture were determined by Fourier transform infrared spectroscopy. OCP tended to be converted to carbonate hydroxyapatite after incubation with or without pulp cells, which may be analogous to biological apatite crystals. Taken together, our data suggest that synthetic OCP supports RD formation by dental pulp and downregulation of Runx2 may be involved in that stimulatory activity. Furthermore, OCP-apatite conversion is involved in this stimulatory capacity of OCP.

Original languageEnglish
Pages (from-to)1310-1320
Number of pages11
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 1 2015

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Odontoblasts
Dentin
Phosphates
Pulp
Dental Pulp
Apatites
Down-Regulation
Apatite
octacalcium phosphate
Biomineralization
Transcription factors
Carbonates
Phosphatases
Biocompatible Materials
Fourier Transform Infrared Spectroscopy
Durapatite
Hydroxyapatite
Biomaterials
Fourier transform infrared spectroscopy
Alkaline Phosphatase

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Synthetic octacalcium phosphate-enhanced reparative dentine formation via induction of odontoblast differentiation. / Wang, Xiaogu; Suzawa, Tetsuo; Miyauchi, Tomohiko; Zhao, Baohong; Yasuhara, Rika; Anada, Takahisa; Nakamura, Masanori; Suzuki, Osamu; Kamijo, Ryutaro.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 9, No. 11, 01.11.2015, p. 1310-1320.

Research output: Contribution to journalArticle

Wang, Xiaogu ; Suzawa, Tetsuo ; Miyauchi, Tomohiko ; Zhao, Baohong ; Yasuhara, Rika ; Anada, Takahisa ; Nakamura, Masanori ; Suzuki, Osamu ; Kamijo, Ryutaro. / Synthetic octacalcium phosphate-enhanced reparative dentine formation via induction of odontoblast differentiation. In: Journal of Tissue Engineering and Regenerative Medicine. 2015 ; Vol. 9, No. 11. pp. 1310-1320.
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