Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide

Zhiyang Zeng, Yu Hoshino, Andy Rodriguez, Hoseong Yoo, Kenneth J. Shea

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide have been prepared by inverse microemulsion polymerization. Peptide affinity was achieved in part by incorporating the target (imprint) peptide in the polymerization reaction mixture. Incorporation of the imprint peptide assists in the creation of complementary binding sites in the resulting polymer nanoparticle (NP). To orient the imprint peptide at the interface of the water and oil domains during polymerization, the peptide target was coupled with fatty acid chains of varying length. The peptide-NP binding affinities (ca. 90-900 nM) were quantitatively evaluated by a quartz crystal microbalance (QCM). The optimal chain length was established that created high affinity peptide binding sites on the surface of the nanoparticles. This method can be used for the preparation of nanosized synthetic polymers with antibody-like affinity for hydrophilic peptides and proteins ("plastic antibodies").

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalACS Nano
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 26 2010
Externally publishedYes

Fingerprint

antibodies
Antibodies
Peptides
peptides
affinity
Polymers
Nanoparticles
nanoparticles
polymers
polymerization
Polymerization
Binding sites
Binding Sites
Quartz crystal microbalances
Microemulsions
fatty acids
quartz crystals
Chain length
Fatty acids
microbalances

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide. / Zeng, Zhiyang; Hoshino, Yu; Rodriguez, Andy; Yoo, Hoseong; Shea, Kenneth J.

In: ACS Nano, Vol. 4, No. 1, 26.01.2010, p. 199-204.

Research output: Contribution to journalArticle

Zeng, Zhiyang ; Hoshino, Yu ; Rodriguez, Andy ; Yoo, Hoseong ; Shea, Kenneth J. / Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide. In: ACS Nano. 2010 ; Vol. 4, No. 1. pp. 199-204.
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