Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide

Zhiyang Zeng, Yu Hoshino, Andy Rodriguez, Hoseong Yoo, Kenneth J. Shea

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide have been prepared by inverse microemulsion polymerization. Peptide affinity was achieved in part by incorporating the target (imprint) peptide in the polymerization reaction mixture. Incorporation of the imprint peptide assists in the creation of complementary binding sites in the resulting polymer nanoparticle (NP). To orient the imprint peptide at the interface of the water and oil domains during polymerization, the peptide target was coupled with fatty acid chains of varying length. The peptide-NP binding affinities (ca. 90-900 nM) were quantitatively evaluated by a quartz crystal microbalance (QCM). The optimal chain length was established that created high affinity peptide binding sites on the surface of the nanoparticles. This method can be used for the preparation of nanosized synthetic polymers with antibody-like affinity for hydrophilic peptides and proteins ("plastic antibodies").

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalACS nano
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 26 2010

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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