TY - JOUR
T1 - Synthetic Strategies for Artificial Lipidation of Functional Proteins
AU - Takahara, Mari
AU - Kamiya, Noriho
N1 - Funding Information:
This work was supported by a Grant‐in‐Aid for Young Scientists 19K15369 (to M.T.) from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan and by the Japan Society for the Promotion of Science (JSPS) KAKENHI No. JP19H00841 (to N.K.). The authors declare no financial or commercial conflict of interest. We thank Renee Mosi, Ph.D., from Edanz Group ( http://www.edanzediting.com/ac ) for editing a draft of this manuscript.
Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/4/9
Y1 - 2020/4/9
N2 - Biosynthesis of natural lipidated proteins is linked to important signal pathways, and therefore analyzing protein lipidation is crucial for understanding cellular functions. Artificial lipidation of proteins has attracted attention in recent decades as it allows modulation of the amphiphilic nature of the protein of interest, and is used in the design of drug-delivery systems containing antibodies anchored on a lipid bilayer carrier. However, the intrinsic hydrophobicity of lipids makes the synthesis of lipid–protein conjugates challenging with respect to the yield and selectivity of the lipidation. In this Minireview, the development of chemical and enzymatic synthetic strategies for the preparation of a range of lipid–protein conjugates that do not compromise the functions of the proteins are discussed as well as applications of the conjugates.
AB - Biosynthesis of natural lipidated proteins is linked to important signal pathways, and therefore analyzing protein lipidation is crucial for understanding cellular functions. Artificial lipidation of proteins has attracted attention in recent decades as it allows modulation of the amphiphilic nature of the protein of interest, and is used in the design of drug-delivery systems containing antibodies anchored on a lipid bilayer carrier. However, the intrinsic hydrophobicity of lipids makes the synthesis of lipid–protein conjugates challenging with respect to the yield and selectivity of the lipidation. In this Minireview, the development of chemical and enzymatic synthetic strategies for the preparation of a range of lipid–protein conjugates that do not compromise the functions of the proteins are discussed as well as applications of the conjugates.
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U2 - 10.1002/chem.201904568
DO - 10.1002/chem.201904568
M3 - Review article
C2 - 31793677
AN - SCOPUS:85078675835
VL - 26
SP - 4645
EP - 4655
JO - Chemistry - A European Journal
JF - Chemistry - A European Journal
SN - 0947-6539
IS - 21
ER -