Systemic oxidative stress is associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients withmetabolic syndrome

Takashi Yokota, Shintaro Kinugawa, Mayumi Yamato, Kagami Hirabayashi, Tadashi Suga, Shingo Takada, Kuniaki Harada, Noriteru Morita, Noriko Oyama-Manabe, Yasuka Kikuchi, Koichi Okita, Hiroyuki Tsutsui

Research output: Contribution to journalArticle

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Abstract

OBJECTIVE-Systemic oxidative stress is associated with insulin resistance and obesity. We tested the hypothesis that systemic oxidative stress is linked to lower aerobic capacity and skeletal muscle dysfunction in metabolic syndrome (MetS). RESEARCH DESIGN AND METHODS-The incremental exercise testing with cycle ergometer was performed in 14 male patients with MetS and 13 age-, sex-, and activity-matched healthy subjects. Systemic lipid peroxidation was assessed by serum thiobarbituric acid reactive substances (TBARS), and systemic antioxidant defense capacity was assessed by serum total thiols and enzymatic activity of superoxide dismutase (SOD). To assess skeletal muscle energy metabolism, we measured high-energy phosphates in the calf muscle during plantar flexion exercise and intramyocellular lipid (IMCL) in the resting leg muscle, using 31P- and 1protonmagnetic resonance spectroscopy, respectively. RESULTS-Serum TBARS were elevated (12.4 ± 7.1 vs. 3.7 ± 1.1 μmol/L; P < 0.01), and serum total thiols and SOD activity were decreased (290.8 ± 51.2 vs. 398.7 ± 105.2 μmol/L, P < 0.01; and 22.2 ± 8.4 vs. 31.5 ± 8.5 units/L, P < 0.05, respectively) in patients with MetS compared with healthy subjects. Peak VO2 and anaerobic threshold normalized to body weight were significantly lower in MetS patients by 25 and 31%, respectively, and inversely correlated with serum TBARS (r = 20.49 and r = 20.50, respectively). Moreover, muscle phosphocreatine loss during exercise was 1.4-fold greater in patients with MetS (P,0.05), and IMCL content was 2.9-fold higher in patients with MetS (P < 0.01), indicating impaired skeletal muscle energy metabolism, and these indices positively correlated with serum TBARS (r = 0.45 and r = 0.63, respectively). CONCLUSIONS-Systemic oxidative stress was associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients with MetS.

Original languageEnglish
Pages (from-to)1341-1346
Number of pages6
JournalDiabetes care
Volume36
Issue number5
DOIs
Publication statusPublished - May 1 2013

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Energy Metabolism
Skeletal Muscle
Oxidative Stress
Thiobarbituric Acid Reactive Substances
Serum
Exercise
Sulfhydryl Compounds
Muscles
Superoxide Dismutase
Healthy Volunteers
Anaerobic Threshold
Lipids
Phosphocreatine
Lipid Peroxidation
Insulin Resistance
Spectrum Analysis
Leg
Research Design
Obesity
Antioxidants

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Advanced and Specialised Nursing

Cite this

Systemic oxidative stress is associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients withmetabolic syndrome. / Yokota, Takashi; Kinugawa, Shintaro; Yamato, Mayumi; Hirabayashi, Kagami; Suga, Tadashi; Takada, Shingo; Harada, Kuniaki; Morita, Noriteru; Oyama-Manabe, Noriko; Kikuchi, Yasuka; Okita, Koichi; Tsutsui, Hiroyuki.

In: Diabetes care, Vol. 36, No. 5, 01.05.2013, p. 1341-1346.

Research output: Contribution to journalArticle

Yokota, T, Kinugawa, S, Yamato, M, Hirabayashi, K, Suga, T, Takada, S, Harada, K, Morita, N, Oyama-Manabe, N, Kikuchi, Y, Okita, K & Tsutsui, H 2013, 'Systemic oxidative stress is associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients withmetabolic syndrome', Diabetes care, vol. 36, no. 5, pp. 1341-1346. https://doi.org/10.2337/dc12-1161
Yokota, Takashi ; Kinugawa, Shintaro ; Yamato, Mayumi ; Hirabayashi, Kagami ; Suga, Tadashi ; Takada, Shingo ; Harada, Kuniaki ; Morita, Noriteru ; Oyama-Manabe, Noriko ; Kikuchi, Yasuka ; Okita, Koichi ; Tsutsui, Hiroyuki. / Systemic oxidative stress is associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients withmetabolic syndrome. In: Diabetes care. 2013 ; Vol. 36, No. 5. pp. 1341-1346.
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abstract = "OBJECTIVE-Systemic oxidative stress is associated with insulin resistance and obesity. We tested the hypothesis that systemic oxidative stress is linked to lower aerobic capacity and skeletal muscle dysfunction in metabolic syndrome (MetS). RESEARCH DESIGN AND METHODS-The incremental exercise testing with cycle ergometer was performed in 14 male patients with MetS and 13 age-, sex-, and activity-matched healthy subjects. Systemic lipid peroxidation was assessed by serum thiobarbituric acid reactive substances (TBARS), and systemic antioxidant defense capacity was assessed by serum total thiols and enzymatic activity of superoxide dismutase (SOD). To assess skeletal muscle energy metabolism, we measured high-energy phosphates in the calf muscle during plantar flexion exercise and intramyocellular lipid (IMCL) in the resting leg muscle, using 31P- and 1protonmagnetic resonance spectroscopy, respectively. RESULTS-Serum TBARS were elevated (12.4 ± 7.1 vs. 3.7 ± 1.1 μmol/L; P < 0.01), and serum total thiols and SOD activity were decreased (290.8 ± 51.2 vs. 398.7 ± 105.2 μmol/L, P < 0.01; and 22.2 ± 8.4 vs. 31.5 ± 8.5 units/L, P < 0.05, respectively) in patients with MetS compared with healthy subjects. Peak VO2 and anaerobic threshold normalized to body weight were significantly lower in MetS patients by 25 and 31{\%}, respectively, and inversely correlated with serum TBARS (r = 20.49 and r = 20.50, respectively). Moreover, muscle phosphocreatine loss during exercise was 1.4-fold greater in patients with MetS (P,0.05), and IMCL content was 2.9-fold higher in patients with MetS (P < 0.01), indicating impaired skeletal muscle energy metabolism, and these indices positively correlated with serum TBARS (r = 0.45 and r = 0.63, respectively). CONCLUSIONS-Systemic oxidative stress was associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients with MetS.",
author = "Takashi Yokota and Shintaro Kinugawa and Mayumi Yamato and Kagami Hirabayashi and Tadashi Suga and Shingo Takada and Kuniaki Harada and Noriteru Morita and Noriko Oyama-Manabe and Yasuka Kikuchi and Koichi Okita and Hiroyuki Tsutsui",
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T1 - Systemic oxidative stress is associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients withmetabolic syndrome

AU - Yokota, Takashi

AU - Kinugawa, Shintaro

AU - Yamato, Mayumi

AU - Hirabayashi, Kagami

AU - Suga, Tadashi

AU - Takada, Shingo

AU - Harada, Kuniaki

AU - Morita, Noriteru

AU - Oyama-Manabe, Noriko

AU - Kikuchi, Yasuka

AU - Okita, Koichi

AU - Tsutsui, Hiroyuki

PY - 2013/5/1

Y1 - 2013/5/1

N2 - OBJECTIVE-Systemic oxidative stress is associated with insulin resistance and obesity. We tested the hypothesis that systemic oxidative stress is linked to lower aerobic capacity and skeletal muscle dysfunction in metabolic syndrome (MetS). RESEARCH DESIGN AND METHODS-The incremental exercise testing with cycle ergometer was performed in 14 male patients with MetS and 13 age-, sex-, and activity-matched healthy subjects. Systemic lipid peroxidation was assessed by serum thiobarbituric acid reactive substances (TBARS), and systemic antioxidant defense capacity was assessed by serum total thiols and enzymatic activity of superoxide dismutase (SOD). To assess skeletal muscle energy metabolism, we measured high-energy phosphates in the calf muscle during plantar flexion exercise and intramyocellular lipid (IMCL) in the resting leg muscle, using 31P- and 1protonmagnetic resonance spectroscopy, respectively. RESULTS-Serum TBARS were elevated (12.4 ± 7.1 vs. 3.7 ± 1.1 μmol/L; P < 0.01), and serum total thiols and SOD activity were decreased (290.8 ± 51.2 vs. 398.7 ± 105.2 μmol/L, P < 0.01; and 22.2 ± 8.4 vs. 31.5 ± 8.5 units/L, P < 0.05, respectively) in patients with MetS compared with healthy subjects. Peak VO2 and anaerobic threshold normalized to body weight were significantly lower in MetS patients by 25 and 31%, respectively, and inversely correlated with serum TBARS (r = 20.49 and r = 20.50, respectively). Moreover, muscle phosphocreatine loss during exercise was 1.4-fold greater in patients with MetS (P,0.05), and IMCL content was 2.9-fold higher in patients with MetS (P < 0.01), indicating impaired skeletal muscle energy metabolism, and these indices positively correlated with serum TBARS (r = 0.45 and r = 0.63, respectively). CONCLUSIONS-Systemic oxidative stress was associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients with MetS.

AB - OBJECTIVE-Systemic oxidative stress is associated with insulin resistance and obesity. We tested the hypothesis that systemic oxidative stress is linked to lower aerobic capacity and skeletal muscle dysfunction in metabolic syndrome (MetS). RESEARCH DESIGN AND METHODS-The incremental exercise testing with cycle ergometer was performed in 14 male patients with MetS and 13 age-, sex-, and activity-matched healthy subjects. Systemic lipid peroxidation was assessed by serum thiobarbituric acid reactive substances (TBARS), and systemic antioxidant defense capacity was assessed by serum total thiols and enzymatic activity of superoxide dismutase (SOD). To assess skeletal muscle energy metabolism, we measured high-energy phosphates in the calf muscle during plantar flexion exercise and intramyocellular lipid (IMCL) in the resting leg muscle, using 31P- and 1protonmagnetic resonance spectroscopy, respectively. RESULTS-Serum TBARS were elevated (12.4 ± 7.1 vs. 3.7 ± 1.1 μmol/L; P < 0.01), and serum total thiols and SOD activity were decreased (290.8 ± 51.2 vs. 398.7 ± 105.2 μmol/L, P < 0.01; and 22.2 ± 8.4 vs. 31.5 ± 8.5 units/L, P < 0.05, respectively) in patients with MetS compared with healthy subjects. Peak VO2 and anaerobic threshold normalized to body weight were significantly lower in MetS patients by 25 and 31%, respectively, and inversely correlated with serum TBARS (r = 20.49 and r = 20.50, respectively). Moreover, muscle phosphocreatine loss during exercise was 1.4-fold greater in patients with MetS (P,0.05), and IMCL content was 2.9-fold higher in patients with MetS (P < 0.01), indicating impaired skeletal muscle energy metabolism, and these indices positively correlated with serum TBARS (r = 0.45 and r = 0.63, respectively). CONCLUSIONS-Systemic oxidative stress was associated with lower aerobic capacity and impaired skeletal muscle energy metabolism in patients with MetS.

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