Systemic up-regulation of sTNFR2 and IL-6 in Porphyromonas gingivalis pneumonia in mice

Milan Petelin, Koji Naruishi, Nobuyuki Shiomi, Junji Mineshiba, Hideo Arai, Fusanori Nishimura, Shogo Takashiba, Yoji Murayama

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aspiration pneumonia is a common cause of death in older people, and the pathophysiology is a chronic respiratory failure with a mild airway inflammation. In this study, we established a mild inflammatory pneumonia model using Porphyromonas gingivalis (Pg) pathogen-infected mice. It elucidated the effects of Pg-infected pneumonia on proinflammatory cytokines tumor necrosis factor (TNF)-α, interleukin-6 (IL-6), and IL-1β production in both lung tissue and serum. We also elucidated production of soluble (s) TNF receptor (R) s, because TNF-α is considered to be a dominant inflammatory mediator. Lung TNF-α levels significantly increased at 2 h after infection, and rapidly returned to basal level at 24 h. Consistent with increase of TNF-α, remarkable increase of sTNFR2 but not sTNFR1 was detected in lung tissue from 2 to 72 h. Interestingly, sTNFR2/sTNFR1 ratio was significantly enhanced at 2 h in serum. In addition, lung IL-1β and IL-6 levels also significantly increased from 2 to 24 h. Importantly, we found that IL-6 levels in serum reflected its local level. These results may suggest that systemically produced sTNFR2 and IL-6 could be a key role to modulate proinflammatory activities of TNF-α in Pg-induced lung inflammation simulated aspiration pneumonia.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalExperimental and Molecular Pathology
Volume76
Issue number1
DOIs
Publication statusPublished - Jan 1 2004
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Interleukin-6
Pneumonia
Up-Regulation
Tumor Necrosis Factor-alpha
Aspiration Pneumonia
Lung
Interleukin-1
Serum
Tissue
Tumor Necrosis Factor Receptors
Pathogens
Respiratory Insufficiency
Cause of Death
Cytokines
Inflammation
Infection

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Systemic up-regulation of sTNFR2 and IL-6 in Porphyromonas gingivalis pneumonia in mice. / Petelin, Milan; Naruishi, Koji; Shiomi, Nobuyuki; Mineshiba, Junji; Arai, Hideo; Nishimura, Fusanori; Takashiba, Shogo; Murayama, Yoji.

In: Experimental and Molecular Pathology, Vol. 76, No. 1, 01.01.2004, p. 76-81.

Research output: Contribution to journalArticle

Petelin, M, Naruishi, K, Shiomi, N, Mineshiba, J, Arai, H, Nishimura, F, Takashiba, S & Murayama, Y 2004, 'Systemic up-regulation of sTNFR2 and IL-6 in Porphyromonas gingivalis pneumonia in mice', Experimental and Molecular Pathology, vol. 76, no. 1, pp. 76-81. https://doi.org/10.1016/j.yexmp.2003.09.002
Petelin, Milan ; Naruishi, Koji ; Shiomi, Nobuyuki ; Mineshiba, Junji ; Arai, Hideo ; Nishimura, Fusanori ; Takashiba, Shogo ; Murayama, Yoji. / Systemic up-regulation of sTNFR2 and IL-6 in Porphyromonas gingivalis pneumonia in mice. In: Experimental and Molecular Pathology. 2004 ; Vol. 76, No. 1. pp. 76-81.
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