T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma

Ikumi Imajyo, Tsuyoshi Sugiura, Yosuke Kobayashi, Miyuki Shimoda, Kotaro Ishii, Naonari Akimoto, Naoya Yoshihama, Ieyoshi Kobayashi, Yoshihide Mori

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The prognosis of patients with oral squamous cell carcinoma (SCC) is influenced by the presence of lymph node metastasis. Epithelial-mesenchymal transition (EMT), a process that involves events that convert adherent epithelial cells into individual migratory cells that can invade the extracellular matrix, is critical for cancer progression. Recently, the T-box transcription factor Brachyury was reported to promote EMT in human carcinoma cell lines. We analyzed the relationship between EMT (assessed by staining for E-cadherin and Vimentin) and the expression of Brachyury in association with lymph node metastasis in oral SCC. Oral SCC biopsy specimens (152 cases) were examined immunohistochemically for the expression of E-cadherin, Vimentin and Brachyury. Expression of Brachyury was correlated with EMT (p=0.035) and was significantly associated with lymph node and distant metastasis (p<0.05). Logistic regression analysis showed that Brachyury and EMT were predictive factors for lymph node metastasis (odds ratio 4.390 and 5.936, respectively) and that EMT was a predictive factor for distant metastases (odds ratio 11.786). Our findings present clinical evidence for an important role of Brachyury in EMT in oral SCC, and suggest that Brachyury and EMT patterns are useful prognostic markers.

Original languageEnglish
Pages (from-to)1985-1995
Number of pages11
JournalInternational journal of oncology
Volume41
Issue number6
DOIs
Publication statusPublished - Dec 1 2012

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Epithelial-Mesenchymal Transition
Squamous Cell Carcinoma
Transcription Factors
Lymph Nodes
Neoplasm Metastasis
Vimentin
Cadherins
Odds Ratio
Brachyury protein
Extracellular Matrix
Logistic Models
Epithelial Cells
Regression Analysis
Staining and Labeling
Carcinoma
Biopsy
Cell Line

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma. / Imajyo, Ikumi; Sugiura, Tsuyoshi; Kobayashi, Yosuke; Shimoda, Miyuki; Ishii, Kotaro; Akimoto, Naonari; Yoshihama, Naoya; Kobayashi, Ieyoshi; Mori, Yoshihide.

In: International journal of oncology, Vol. 41, No. 6, 01.12.2012, p. 1985-1995.

Research output: Contribution to journalArticle

Imajyo, Ikumi ; Sugiura, Tsuyoshi ; Kobayashi, Yosuke ; Shimoda, Miyuki ; Ishii, Kotaro ; Akimoto, Naonari ; Yoshihama, Naoya ; Kobayashi, Ieyoshi ; Mori, Yoshihide. / T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma. In: International journal of oncology. 2012 ; Vol. 41, No. 6. pp. 1985-1995.
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