T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles

Akira Ito, Masaki Yamaguchi, Noriaki Okamoto, Yuji Sanematsu, Kawabe Yoshinori, Kazumasa Wakamatsu, Shosuke Ito, Hiroyuki Honda, Takeshi Kobayashi, Eiichi Nakayama, Yasuaki Tamura, Masae Okura, Toshiharu Yamashita, Kowichi Jimbow, Masamichi Kamihira

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aim: Accumulating evidence has indicated that hyperthermia using magnetite nanoparticles induces antitumor immunity. This study investigated the diversity of T-cell receptors (TCRs) in tumor-infiltrating lymphocytes after hyperthermia using magnetite nanoparticles.Materials & methods: Functionalized magnetite nanoparticles, N-propionyl-4-S-cysteaminylphenol (NPrCAP)/magnetite, were synthesized by conjugating the melanogenesis substrate NPrCAP with magnetite nanoparticles. NPrCAP/magnetite nanoparticles were injected into B16 melanomas in C57BL/6 mice, which were subjected to an alternating magnetic field for hyperthermia treatment. Results: Enlargement of the tumor-draining lymph nodes was observed after hyperthermia. The TCR repertoire was restricted in tumor-infiltrating lymphocytes, and expansion of Vβ11+ T cells was preferentially found. DNA sequences of the third complementaritydetermining regions revealed the presence of clonally expanded T cells. Conclusion: These results indicate that the T-cell response in B16 melanomas after hyperthermia is dominated by T cells directed toward a limited number of epitopes and that epitope-specific T cells frequently use a restricted TCR repertoire.

Original languageEnglish
Pages (from-to)891-902
Number of pages12
JournalNanomedicine
Volume8
Issue number6
DOIs
Publication statusPublished - Jan 1 2013

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Development

Fingerprint Dive into the research topics of 'T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles'. Together they form a unique fingerprint.

  • Cite this

    Ito, A., Yamaguchi, M., Okamoto, N., Sanematsu, Y., Yoshinori, K., Wakamatsu, K., Ito, S., Honda, H., Kobayashi, T., Nakayama, E., Tamura, Y., Okura, M., Yamashita, T., Jimbow, K., & Kamihira, M. (2013). T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles. Nanomedicine, 8(6), 891-902. https://doi.org/10.2217/nnm.12.142