T-cell receptors from virus-specific cytotoxic T lymphocytes recognizing a single immunodominant nine-amino-acid viral epitope show marked diversity

M. S. Horwitz, Yusuke Yanagi, M. B.A. Oldstone

Research output: Contribution to journalArticle

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Abstract

Following infection of the H-2(d) mouse by lymphocytic choriomeningitis virus, the newly generated cytotoxic T lymphocyte (CTL) response is focused to a single 9-amino-acid peptide sequence (epitope) of the virus. More than 96% of the primary, secondary, and clonal CTL respond to this lymphocytic choriomeningitis virus nucleoprotein epitope. This unique system affords the opportunity to evaluate the T-cell response to a single viral CTL epitope in a case in which the outcome of infection, either viral clearance or host death, is mediated by the CTLs. Specifically, the molecular structure of the T-cell receptors (TCRs) of CTLs responding to this epitope was analyzed. By using an anchored polymerase chain reaction, the TCR chains of three CTL clones cDNAs were amplified, sequenced, and found to have unique V(α) or V(β) chains relative to each other as well as to lack restriction to any particular variable chain. These data indicate that the highly diverse antiviral CTL response is pleomorphic and probably provides an advantage to the host as it limits the emergence of viral variants that could more easily arise if the TCR response were homogeneous.

Original languageEnglish
Pages (from-to)352-357
Number of pages6
JournalJournal of Virology
Volume68
Issue number1
Publication statusPublished - Jan 1 1994
Externally publishedYes

Fingerprint

cytotoxic T-lymphocytes
Cytotoxic T-Lymphocytes
T-Cell Antigen Receptor
epitopes
Epitopes
T-lymphocytes
Viruses
Amino Acids
viruses
receptors
Lymphocytic choriomeningitis virus
amino acids
nucleoproteins
Nucleoproteins
T-Lymphocyte Epitopes
Virus Diseases
Molecular Structure
chemical structure
infection
Antiviral Agents

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

T-cell receptors from virus-specific cytotoxic T lymphocytes recognizing a single immunodominant nine-amino-acid viral epitope show marked diversity. / Horwitz, M. S.; Yanagi, Yusuke; Oldstone, M. B.A.

In: Journal of Virology, Vol. 68, No. 1, 01.01.1994, p. 352-357.

Research output: Contribution to journalArticle

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