T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis

Hideki Ohyama, Sho Matsushita, Nahoko Kato, Fusanori Nishimura, Kosuke Oyaizu, Susumu Kokeguchi, Hidemi Kurihara, Shogo Takashiba, Yasuharu Nishimura, Yoji Murayama

Research output: Contribution to journalArticle

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Abstract

Patients with early-onset periodontitis (EOP) are susceptible to infection with periodontopathic bacteria, such as Porphyromonas gingivalis. Ag53, 53-kDa outer membrane protein of P. gingivalis, evokes strong humoral immune responses in EOP patients. In a first step to clarify how host immune cells recognize Ag53, we established Ag53-specific short-term T cell lines from 22 subjects including 6 EOP patients and 16 healthy donors, using overlapping peptides based on Ag53 amino acid sequences. All T cell lines from active EOP patients recognized a common region (p141-181, especially p141-161) on Ag53, while those from healthy donors showed heterogeneous specificity. p141-181 was not recognized by T cell lines established from EOP patients following therapy. A monoclonal antibody to HLA-DRB1 inhibited Ag53- induced proliferation of most of the T cell lines. Our observations suggest that, although antigen-presenting molecules are common in EOP patients and in healthy individuals, p141-161 includes a major T cell epitope(s) on Ag53 for active EOP patients but not for healthy individuals or inactive EOP patients.

Original languageEnglish
Pages (from-to)635-643
Number of pages9
JournalHuman Immunology
Volume59
Issue number10
DOIs
Publication statusPublished - Oct 1 1998
Externally publishedYes

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Aggressive Periodontitis
Porphyromonas gingivalis
Membrane Proteins
T-Lymphocytes
Cell Line
Tissue Donors
HLA-DRB1 Chains
T-Lymphocyte Epitopes
Humoral Immunity
Amino Acid Sequence
Monoclonal Antibodies
Bacteria
Antigens
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis. / Ohyama, Hideki; Matsushita, Sho; Kato, Nahoko; Nishimura, Fusanori; Oyaizu, Kosuke; Kokeguchi, Susumu; Kurihara, Hidemi; Takashiba, Shogo; Nishimura, Yasuharu; Murayama, Yoji.

In: Human Immunology, Vol. 59, No. 10, 01.10.1998, p. 635-643.

Research output: Contribution to journalArticle

Ohyama, H, Matsushita, S, Kato, N, Nishimura, F, Oyaizu, K, Kokeguchi, S, Kurihara, H, Takashiba, S, Nishimura, Y & Murayama, Y 1998, 'T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis', Human Immunology, vol. 59, no. 10, pp. 635-643. https://doi.org/10.1016/S0198-8859(98)00064-0
Ohyama, Hideki ; Matsushita, Sho ; Kato, Nahoko ; Nishimura, Fusanori ; Oyaizu, Kosuke ; Kokeguchi, Susumu ; Kurihara, Hidemi ; Takashiba, Shogo ; Nishimura, Yasuharu ; Murayama, Yoji. / T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis. In: Human Immunology. 1998 ; Vol. 59, No. 10. pp. 635-643.
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