T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy

Hideki Ohyama, Sho Matsushita, Fusanori Nishimura, Nahoko Kato, Kentaro Hatano, Shogo Takashiba, Yoji Murayama

Research output: Contribution to journalArticle

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Abstract

Major membrane protein II (MMP II) of Mycobacterium leprae (M. leprae) is a 22kDa protein inducing humoral immune response in leprosy patients. MMP II-specific bulk T cell lines were established from leprosy patients to determine major T cell epitopes in MMP II and to evaluate lymphokine production induced by MMP II. These bulk T cell lines reacted to one or more peptides in the locus of amino acid residues from 23 to 109 of MMP II. The proliferative responses of all T cell lines were mainly inhibited by the addition of anti-DRB1 mAb. Many bulk T cell lines induced IFN-γ, IL-5, but not IL-4. However, it was not possible to distinguish the LL or TT types of leprosy based on the pattern of T cell epitopes and the lymphokine productivity in the responses against MMP II. Thus, it appears that T cell response to MMP II is restricted by the HLA-DRB1 molecule, but not by DQ and DP molecules, which results in the induction of IFN-γ production.

Original languageEnglish
Pages (from-to)475-482
Number of pages8
JournalVaccine
Volume20
Issue number3-4
DOIs
Publication statusPublished - Nov 12 2001
Externally publishedYes

Fingerprint

Mycobacterium leprae
leprosy
HLA-DR Antigens
Leprosy
membrane proteins
Membrane Proteins
T-lymphocytes
T-Lymphocytes
Cell Line
cell lines
T-Lymphocyte Epitopes
lymphokines
Lymphokines
epitopes
HLA-DRB1 Chains
Interleukin-5
interleukin-5
Humoral Immunity
Interleukin-4
interleukin-4

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy. / Ohyama, Hideki; Matsushita, Sho; Nishimura, Fusanori; Kato, Nahoko; Hatano, Kentaro; Takashiba, Shogo; Murayama, Yoji.

In: Vaccine, Vol. 20, No. 3-4, 12.11.2001, p. 475-482.

Research output: Contribution to journalArticle

Ohyama, Hideki ; Matsushita, Sho ; Nishimura, Fusanori ; Kato, Nahoko ; Hatano, Kentaro ; Takashiba, Shogo ; Murayama, Yoji. / T cell responses to major membrane protein II (MMP II) of Mycobacterium leprae are restricted by HLA-DR molecules in patients with leprosy. In: Vaccine. 2001 ; Vol. 20, No. 3-4. pp. 475-482.
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