Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers

Satomi Yoshimura, Atsushi Yoshimura, Nobuo Iwata, Susan R. McCouch, M. Lleva Abenes, Marietta R. Baraoidan, Twng Wah Mew, Rebecca J. Nelson

Research output: Contribution to journalArticle

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Abstract

Four genes of rice, Oryza sativa L., conditioning resistance to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae), were tagged by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. No recombinants were observed between xa-5 and RFLP marker loci RZ390, RG556 or RG207 on chromosome 5. Xa-3 and Xa-4 were linked to RFLP locus XNpb181 at the top of chromosome 11, at distances of 2.3 cM and 1.7 cM, respectively. The nearest marker to Xa-10, also located on chromosome 11, was the RAPD locus O072000 at a distance of 5.3 cM. From this study, the conventional map [19, 28] and two RFLP linkage maps of chromosome 11 [14, 26] were partially integrated. Using the RFLP and RAPD markers linked to the resistance genes, we selected rice lines homozygous for pairs of resistance genes, Xa-4 +xa-5 and Xa-4 +Xa-10. Lines carrying Xa-4 +xa-5 and Xa-4 +Xa-10 were evaluated for reaction to eight strains of the bacterial blight pathogen, representing eight pathotypes and three genetic lineages. As expected, the lines carrying pairs of genes were resistant to more of the isolates than their single-gene parental lines. Lines carrying Xa-4 +xa-5 were more resistant to isolates of race 4 than were either of the parental lines ('quantitative complementation'). No such effects were seen for Xa-4 +Xa-10. Thus, combinations of resistance genes provide broader spectra of resistance through both ordinary gene action expected and quantitative complementation.

Original languageEnglish
Pages (from-to)375-387
Number of pages13
JournalMolecular Breeding
Volume1
Issue number4
DOIs
Publication statusPublished - Dec 1 1995

Fingerprint

random amplified polymorphic DNA technique
blight
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
rice
DNA
Chromosomes, Human, Pair 11
Genes
genes
chromosomes
Genetic Markers
loci
Xanthomonas
Xanthomonas oryzae pv. oryzae
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 5
genetic markers
pathogens
Oryza
pathotypes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Yoshimura, S., Yoshimura, A., Iwata, N., McCouch, S. R., Abenes, M. L., Baraoidan, M. R., ... Nelson, R. J. (1995). Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Molecular Breeding, 1(4), 375-387. https://doi.org/10.1007/BF01248415

Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. / Yoshimura, Satomi; Yoshimura, Atsushi; Iwata, Nobuo; McCouch, Susan R.; Abenes, M. Lleva; Baraoidan, Marietta R.; Mew, Twng Wah; Nelson, Rebecca J.

In: Molecular Breeding, Vol. 1, No. 4, 01.12.1995, p. 375-387.

Research output: Contribution to journalArticle

Yoshimura, S, Yoshimura, A, Iwata, N, McCouch, SR, Abenes, ML, Baraoidan, MR, Mew, TW & Nelson, RJ 1995, 'Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers', Molecular Breeding, vol. 1, no. 4, pp. 375-387. https://doi.org/10.1007/BF01248415
Yoshimura S, Yoshimura A, Iwata N, McCouch SR, Abenes ML, Baraoidan MR et al. Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Molecular Breeding. 1995 Dec 1;1(4):375-387. https://doi.org/10.1007/BF01248415
Yoshimura, Satomi ; Yoshimura, Atsushi ; Iwata, Nobuo ; McCouch, Susan R. ; Abenes, M. Lleva ; Baraoidan, Marietta R. ; Mew, Twng Wah ; Nelson, Rebecca J. / Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. In: Molecular Breeding. 1995 ; Vol. 1, No. 4. pp. 375-387.
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abstract = "Four genes of rice, Oryza sativa L., conditioning resistance to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae), were tagged by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) markers. No recombinants were observed between xa-5 and RFLP marker loci RZ390, RG556 or RG207 on chromosome 5. Xa-3 and Xa-4 were linked to RFLP locus XNpb181 at the top of chromosome 11, at distances of 2.3 cM and 1.7 cM, respectively. The nearest marker to Xa-10, also located on chromosome 11, was the RAPD locus O072000 at a distance of 5.3 cM. From this study, the conventional map [19, 28] and two RFLP linkage maps of chromosome 11 [14, 26] were partially integrated. Using the RFLP and RAPD markers linked to the resistance genes, we selected rice lines homozygous for pairs of resistance genes, Xa-4 +xa-5 and Xa-4 +Xa-10. Lines carrying Xa-4 +xa-5 and Xa-4 +Xa-10 were evaluated for reaction to eight strains of the bacterial blight pathogen, representing eight pathotypes and three genetic lineages. As expected, the lines carrying pairs of genes were resistant to more of the isolates than their single-gene parental lines. Lines carrying Xa-4 +xa-5 were more resistant to isolates of race 4 than were either of the parental lines ('quantitative complementation'). No such effects were seen for Xa-4 +Xa-10. Thus, combinations of resistance genes provide broader spectra of resistance through both ordinary gene action expected and quantitative complementation.",
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