TY - JOUR
T1 - TAK1 adaptor proteins, TAB2 and TAB3, link the signalosome to B-cell receptor-induced IKK activation
AU - Shinohara, Hisaaki
AU - Yasuda, Tomoharu
AU - Kurosaki, Tomohiro
N1 - Funding Information:
This research was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (nos. 15K08534 and 15H01195); the Mochida Memorial Foundation; and the Takeda Science Foundation (to H.S).
Publisher Copyright:
© 2016 Federation of European Biochemical Societies
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Transforming growth factor-β-activated kinase (TAK)1-binding proteins (TAB) activate nuclear factor-κB by linking TAK1 to signaling molecules. We investigated the mechanisms underlying B-cell receptor (BCR) signaling in TAB2- and TAB3-deficient and TAB3 domain deletion mutant DT40 B cell lines. Loss of TAB2 and TAB3 abolished BCR-induced inhibitor of κB kinase (IKK) activation and TAK1 binding to caspase recruitment domain membrane-associated guanylate kinase protein (CARMA)1. Deletion of TAB3, coupling of ubiquitin conjugation to ER degradation, coiled-coil, and zinc finger domains blocked IKK activation and association with CARMA1. Thus, TAB2 and TAB3 connect signaling molecules that activate IKK in BCR signaling.
AB - Transforming growth factor-β-activated kinase (TAK)1-binding proteins (TAB) activate nuclear factor-κB by linking TAK1 to signaling molecules. We investigated the mechanisms underlying B-cell receptor (BCR) signaling in TAB2- and TAB3-deficient and TAB3 domain deletion mutant DT40 B cell lines. Loss of TAB2 and TAB3 abolished BCR-induced inhibitor of κB kinase (IKK) activation and TAK1 binding to caspase recruitment domain membrane-associated guanylate kinase protein (CARMA)1. Deletion of TAB3, coupling of ubiquitin conjugation to ER degradation, coiled-coil, and zinc finger domains blocked IKK activation and association with CARMA1. Thus, TAB2 and TAB3 connect signaling molecules that activate IKK in BCR signaling.
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U2 - 10.1002/1873-3468.12342
DO - 10.1002/1873-3468.12342
M3 - Article
C2 - 27497262
AN - SCOPUS:84982231246
SN - 0014-5793
VL - 590
SP - 3264
EP - 3269
JO - FEBS Letters
JF - FEBS Letters
IS - 18
ER -