Tal1/Scl gene transduction using a lentiviral vector stimulates highly efficient hematopoietic cell differentiation from common marmoset (Callithrix jacchus) embryonic stem cells

Ryo Kurita, Erika Sasaki, Tomoko Yokoo, Takashi Hiroyama, Kashiya Takasugi, Hideyuki Imoto, Kiyoko Izawa, Yan Dong, Takao Hashiguchi, Yasushi Soda, Toyoki Maeda, Youko Suehiro, Yoshikuni Tanioka, Yukoh Nakazaki, Kenzaburo Tani

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22 Citations (Scopus)

Abstract

The development of embryonic stem cell (ESC) therapies requires the establishment of efficient methods to differentiate ESCs into specific cell lineages. Here, we report the in vitro differentiation of common marmoset (CM) (Callithrix jacchus) ESCs into hematopoietic cells after exogenous gene transfer using vesicular stomatitis virus-glycoprotein-pseudotyped lentiviral vectors. We transduced hematopoietic genes, including tal1/scl, gata1, gata2, hoxB4, and lhx2, into CM ESCs. By immunochemical and morphological analyses, we demonstrated that overexpression of tal1/scl, but not the remaining genes, dramatically increased hematopoiesis of CM ESCs, resulting in multiple blood-cell lineages. Furthermore, flow cytometric analysis demonstrated that CD34, a hematopoietic stem/progenitor cell marker, was highly expressed in tal1/scl-overexpressing embryoid body cells. Similar results were obtained from three independent CM ESC lines. These results suggest that transduction of exogenous tal1/scl cDNA into ESCs is a promising method to induce the efficient differentiation of CM ESCs into hematopoietic stem/progenitor cells.

Original languageEnglish
Pages (from-to)2014-2022
Number of pages9
JournalSTEM CELLS
Volume24
Issue number9
DOIs
Publication statusPublished - Oct 5 2006

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All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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