TaqMan real-time polymerase chain reaction assay for the correlation of Treponema denticola numbers with the severity of periodontal disease

A. Yoshida, M. Kawada, N. Suzuki, Y. Nakano, T. Oho, T. Saito, Yoshihisa Yamashita

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

Treponema denticola has been implicated in periodontitis, and the presence of this organism in periodontal pockets has been investigated. However, qualitative analysis is insufficient for the clinical evaluation of periodontal treatments, and quantification of T. denticola populations is essential for monitoring therapeutic efficacy. Therefore, we developed a quantitative method for T. denticola that uses the TaqMan real-time polymerase chain reaction assay. Using this system, we evaluated the relative and absolute numbers of this organism in saliva and subgingival plaque. Furthermore, we analyzed the relationship between the numbers of T. denticola and pocket depth, and found a significant positive correlation (P < 0.0001) between these parameters. This report demonstrates the broad potential of real-time polymerase chain reaction applications in periodontology.

Original languageEnglish
Pages (from-to)196-200
Number of pages5
JournalOral Microbiology and Immunology
Volume19
Issue number3
DOIs
Publication statusPublished - Jun 2004

All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

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