TY - JOUR
T1 - TaqMan real-time polymerase chain reaction assay for the correlation of Treponema denticola numbers with the severity of periodontal disease
AU - Yoshida, A.
AU - Kawada, M.
AU - Suzuki, N.
AU - Nakano, Y.
AU - Oho, T.
AU - Saito, T.
AU - Yamashita, Yoshihisa
PY - 2004/6
Y1 - 2004/6
N2 - Treponema denticola has been implicated in periodontitis, and the presence of this organism in periodontal pockets has been investigated. However, qualitative analysis is insufficient for the clinical evaluation of periodontal treatments, and quantification of T. denticola populations is essential for monitoring therapeutic efficacy. Therefore, we developed a quantitative method for T. denticola that uses the TaqMan real-time polymerase chain reaction assay. Using this system, we evaluated the relative and absolute numbers of this organism in saliva and subgingival plaque. Furthermore, we analyzed the relationship between the numbers of T. denticola and pocket depth, and found a significant positive correlation (P < 0.0001) between these parameters. This report demonstrates the broad potential of real-time polymerase chain reaction applications in periodontology.
AB - Treponema denticola has been implicated in periodontitis, and the presence of this organism in periodontal pockets has been investigated. However, qualitative analysis is insufficient for the clinical evaluation of periodontal treatments, and quantification of T. denticola populations is essential for monitoring therapeutic efficacy. Therefore, we developed a quantitative method for T. denticola that uses the TaqMan real-time polymerase chain reaction assay. Using this system, we evaluated the relative and absolute numbers of this organism in saliva and subgingival plaque. Furthermore, we analyzed the relationship between the numbers of T. denticola and pocket depth, and found a significant positive correlation (P < 0.0001) between these parameters. This report demonstrates the broad potential of real-time polymerase chain reaction applications in periodontology.
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U2 - 10.1111/j.0902-0055.2004.00142.x
DO - 10.1111/j.0902-0055.2004.00142.x
M3 - Article
C2 - 15107072
AN - SCOPUS:2442454521
SN - 2041-1006
VL - 19
SP - 196
EP - 200
JO - Molecular Oral Microbiology
JF - Molecular Oral Microbiology
IS - 3
ER -