Abstract
Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.
| Original language | English |
|---|---|
| Pages (from-to) | 283-288 |
| Number of pages | 6 |
| Journal | FEMS microbiology letters |
| Volume | 128 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 15 1995 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
- Genetics
Cite this
Targeted disruption of the PD78 gene (traF) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis. / Nakayama, Jiro; Clewell, Don B.; Suzuki, Akinori.
In: FEMS microbiology letters, Vol. 128, No. 3, 15.05.1995, p. 283-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Targeted disruption of the PD78 gene (traF) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis
AU - Nakayama, Jiro
AU - Clewell, Don B.
AU - Suzuki, Akinori
PY - 1995/5/15
Y1 - 1995/5/15
N2 - Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.
AB - Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029025711&partnerID=8YFLogxK
U2 - 10.1016/0378-1097(95)00118-O
DO - 10.1016/0378-1097(95)00118-O
M3 - Article
C2 - 7781976
AN - SCOPUS:0029025711
VL - 128
SP - 283
EP - 288
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 3
ER -