TY - JOUR
T1 - Targeted disruption of the PD78 gene (traF) reduces pheromone-inducible conjugal transfer of the bacteriocin plasmid pPD1 in Enterococcus faecalis
AU - Nakayama, Jiro
AU - Clewell, Don B.
AU - Suzuki, Akinori
N1 - Funding Information:
We thankT . Sasaki,M . Sulavik and Y. Su for technicaal dviseso n this researcha, ndA . Isogai for helpfuld iscussionT. his work was supportedin part by a Grantf rom Ministryo f EducationS, cience,a nd Cultureo f Japan.
PY - 1995/5/15
Y1 - 1995/5/15
N2 - Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.
AB - Bacterial sex pheromone, cPD1, induces sexual aggregation of Enterococcus faecalis harboring the bacteriocin plasmid, pPD1, and enables pPD1 to transfer at high frequency in a liquid culture. PD78 is a cPD1-inducible cell surface protein encoded by pPD1. The PD78 gene, traF, was disrupted by homologous recombination between pPD1 and an artificial vector having a deletion in the middle portion of traF. The disruption of traF did not affect the cPD1-inducible aggregation but reduced the transfer frequency of pPD1 to 2% of the wild-type level.
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U2 - 10.1016/0378-1097(95)00118-O
DO - 10.1016/0378-1097(95)00118-O
M3 - Article
C2 - 7781976
AN - SCOPUS:0029025711
VL - 128
SP - 283
EP - 288
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 3
ER -