TY - JOUR
T1 - Targeted expression of ced-3 and Ice induces programmed cell death in Drosophila
AU - Shigenaga, Ayako
AU - Funahashi, Yoshimistu
AU - Kimura, Ken Ichi
AU - Kobayakawa, Yoshitaka
AU - Kamada, Shinji
AU - Tsujimoto, Yoshihide
AU - Tanimura, Teiichi
N1 - Funding Information:
We thank BA Hay, Y Hiromi, K Matthews and S Hayashi for providing strains used in this study; SC Fujita for the antibody; N Perrimon for the pUAST vector; Y Oshima for the cDNA library; S Goto for primers. We also thank M Iwasaki for the use of SEM. We thank PE Hardin and IA Meinertzhagen for comments on the manuscript. We are grateful for the technical assistance of I Fukahori, K Tai, M Yamasaki and M Haruta. AS thanks A Matsumoto for valuable discussions and continuous encouragement. This work was supported by Grant-in Aids from the Ministry of Education, Science and Culture of Japan to TT and YT.
PY - 1997
Y1 - 1997
N2 - CED-3 is a cysteine protease required for programmed cell death in the nematode, Caenorhabditis elegans, and shares a sequence similarity with mammalian ICE (interleukin-1β converting enzyme) family proteases. Both CED-3 and ICE family proteases can induce programmed cell death in mammalian cells. Structural and functional similarities between CED-3 and ICE family proteases indicate that the mechanism of cell death is evolutionarily conserved, suggesting the presence of a similar mechanism involving CED-3/ICE-like proteases in Drosophila. Here we determined whether CED-3 or ICE functions to induce programmed cell death in Drosophila. We have generated transformant lines in which ced-3 or Ice is ectopically expressed using the GAL4-UAS system. Expression of CED-3 and ICE can elicit cell death in Drosophila and the cell death was blocked by coexpressing the p35 gene which encodes a viral inhibitor of CED-3/ICE proteases. Results support the idea that the mechanism of programmed cell death controlled by CED-3/ICE is conserved among widely divergent animal species including Drosophila, and the system described provides a tool to dissect cell death mechanism downstream of CED-3/ICE proteases.
AB - CED-3 is a cysteine protease required for programmed cell death in the nematode, Caenorhabditis elegans, and shares a sequence similarity with mammalian ICE (interleukin-1β converting enzyme) family proteases. Both CED-3 and ICE family proteases can induce programmed cell death in mammalian cells. Structural and functional similarities between CED-3 and ICE family proteases indicate that the mechanism of cell death is evolutionarily conserved, suggesting the presence of a similar mechanism involving CED-3/ICE-like proteases in Drosophila. Here we determined whether CED-3 or ICE functions to induce programmed cell death in Drosophila. We have generated transformant lines in which ced-3 or Ice is ectopically expressed using the GAL4-UAS system. Expression of CED-3 and ICE can elicit cell death in Drosophila and the cell death was blocked by coexpressing the p35 gene which encodes a viral inhibitor of CED-3/ICE proteases. Results support the idea that the mechanism of programmed cell death controlled by CED-3/ICE is conserved among widely divergent animal species including Drosophila, and the system described provides a tool to dissect cell death mechanism downstream of CED-3/ICE proteases.
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U2 - 10.1038/sj.cdd.4400254
DO - 10.1038/sj.cdd.4400254
M3 - Article
C2 - 16465256
AN - SCOPUS:9844260582
SN - 1350-9047
VL - 4
SP - 371
EP - 377
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
IS - 5
ER -