Targets and dynamics of promoter DNA methylation during early mouse development

Julie Borgel, Sylvain Guibert, Yufeng Li, Hatsune Chiba, Dirk Schübeler, Hiroyuki Sasaki, Thierry Forné, Michael Weber

    Research output: Contribution to journalArticle

    382 Citations (Scopus)

    Abstract

    DNA methylation is extensively reprogrammed during the early phases of mammalian development, yet genomic targets of this process are largely unknown. We optimized methylated DNA immunoprecipitation for low numbers of cells and profiled DNA methylation during early development of the mouse embryonic lineage in vivo. We observed a major epigenetic switch during implantation at the transition from the blastocyst to the postimplantation epiblast. During this period, DNA methylation is primarily targeted to repress the germline expression program. DNA methylation in the epiblast is also targeted to promoters of lineage-specific genes such as hematopoietic genes, which are subsequently demethylated during terminal differentiation. De novo methylation during early embryogenesis is catalyzed by Dnmt3b, and absence of DNA methylation leads to ectopic gene activation in the embryo. Finally, we identify nonimprinted genes that inherit promoter DNA methylation from parental gametes, suggesting that escape of post-fertilization DNA methylation reprogramming is prevalent in the mouse genome.

    Original languageEnglish
    Pages (from-to)1093-1100
    Number of pages8
    JournalNature genetics
    Volume42
    Issue number12
    DOIs
    Publication statusPublished - Dec 1 2010

    Fingerprint

    DNA Methylation
    Germ Layers
    Embryonic Development
    Genes
    Blastocyst
    Immunoprecipitation
    Fertilization
    Epigenomics
    Germ Cells
    Methylation
    Transcriptional Activation
    Embryonic Structures
    Cell Count
    Genome
    DNA

    All Science Journal Classification (ASJC) codes

    • Genetics

    Cite this

    Borgel, J., Guibert, S., Li, Y., Chiba, H., Schübeler, D., Sasaki, H., ... Weber, M. (2010). Targets and dynamics of promoter DNA methylation during early mouse development. Nature genetics, 42(12), 1093-1100. https://doi.org/10.1038/ng.708

    Targets and dynamics of promoter DNA methylation during early mouse development. / Borgel, Julie; Guibert, Sylvain; Li, Yufeng; Chiba, Hatsune; Schübeler, Dirk; Sasaki, Hiroyuki; Forné, Thierry; Weber, Michael.

    In: Nature genetics, Vol. 42, No. 12, 01.12.2010, p. 1093-1100.

    Research output: Contribution to journalArticle

    Borgel, J, Guibert, S, Li, Y, Chiba, H, Schübeler, D, Sasaki, H, Forné, T & Weber, M 2010, 'Targets and dynamics of promoter DNA methylation during early mouse development', Nature genetics, vol. 42, no. 12, pp. 1093-1100. https://doi.org/10.1038/ng.708
    Borgel, Julie ; Guibert, Sylvain ; Li, Yufeng ; Chiba, Hatsune ; Schübeler, Dirk ; Sasaki, Hiroyuki ; Forné, Thierry ; Weber, Michael. / Targets and dynamics of promoter DNA methylation during early mouse development. In: Nature genetics. 2010 ; Vol. 42, No. 12. pp. 1093-1100.
    @article{7746f5e070904be5a15029093156b4ca,
    title = "Targets and dynamics of promoter DNA methylation during early mouse development",
    abstract = "DNA methylation is extensively reprogrammed during the early phases of mammalian development, yet genomic targets of this process are largely unknown. We optimized methylated DNA immunoprecipitation for low numbers of cells and profiled DNA methylation during early development of the mouse embryonic lineage in vivo. We observed a major epigenetic switch during implantation at the transition from the blastocyst to the postimplantation epiblast. During this period, DNA methylation is primarily targeted to repress the germline expression program. DNA methylation in the epiblast is also targeted to promoters of lineage-specific genes such as hematopoietic genes, which are subsequently demethylated during terminal differentiation. De novo methylation during early embryogenesis is catalyzed by Dnmt3b, and absence of DNA methylation leads to ectopic gene activation in the embryo. Finally, we identify nonimprinted genes that inherit promoter DNA methylation from parental gametes, suggesting that escape of post-fertilization DNA methylation reprogramming is prevalent in the mouse genome.",
    author = "Julie Borgel and Sylvain Guibert and Yufeng Li and Hatsune Chiba and Dirk Sch{\"u}beler and Hiroyuki Sasaki and Thierry Forn{\'e} and Michael Weber",
    year = "2010",
    month = "12",
    day = "1",
    doi = "10.1038/ng.708",
    language = "English",
    volume = "42",
    pages = "1093--1100",
    journal = "Nature Genetics",
    issn = "1061-4036",
    publisher = "Nature Publishing Group",
    number = "12",

    }

    TY - JOUR

    T1 - Targets and dynamics of promoter DNA methylation during early mouse development

    AU - Borgel, Julie

    AU - Guibert, Sylvain

    AU - Li, Yufeng

    AU - Chiba, Hatsune

    AU - Schübeler, Dirk

    AU - Sasaki, Hiroyuki

    AU - Forné, Thierry

    AU - Weber, Michael

    PY - 2010/12/1

    Y1 - 2010/12/1

    N2 - DNA methylation is extensively reprogrammed during the early phases of mammalian development, yet genomic targets of this process are largely unknown. We optimized methylated DNA immunoprecipitation for low numbers of cells and profiled DNA methylation during early development of the mouse embryonic lineage in vivo. We observed a major epigenetic switch during implantation at the transition from the blastocyst to the postimplantation epiblast. During this period, DNA methylation is primarily targeted to repress the germline expression program. DNA methylation in the epiblast is also targeted to promoters of lineage-specific genes such as hematopoietic genes, which are subsequently demethylated during terminal differentiation. De novo methylation during early embryogenesis is catalyzed by Dnmt3b, and absence of DNA methylation leads to ectopic gene activation in the embryo. Finally, we identify nonimprinted genes that inherit promoter DNA methylation from parental gametes, suggesting that escape of post-fertilization DNA methylation reprogramming is prevalent in the mouse genome.

    AB - DNA methylation is extensively reprogrammed during the early phases of mammalian development, yet genomic targets of this process are largely unknown. We optimized methylated DNA immunoprecipitation for low numbers of cells and profiled DNA methylation during early development of the mouse embryonic lineage in vivo. We observed a major epigenetic switch during implantation at the transition from the blastocyst to the postimplantation epiblast. During this period, DNA methylation is primarily targeted to repress the germline expression program. DNA methylation in the epiblast is also targeted to promoters of lineage-specific genes such as hematopoietic genes, which are subsequently demethylated during terminal differentiation. De novo methylation during early embryogenesis is catalyzed by Dnmt3b, and absence of DNA methylation leads to ectopic gene activation in the embryo. Finally, we identify nonimprinted genes that inherit promoter DNA methylation from parental gametes, suggesting that escape of post-fertilization DNA methylation reprogramming is prevalent in the mouse genome.

    UR - http://www.scopus.com/inward/record.url?scp=78649459828&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=78649459828&partnerID=8YFLogxK

    U2 - 10.1038/ng.708

    DO - 10.1038/ng.708

    M3 - Article

    C2 - 21057502

    AN - SCOPUS:78649459828

    VL - 42

    SP - 1093

    EP - 1100

    JO - Nature Genetics

    JF - Nature Genetics

    SN - 1061-4036

    IS - 12

    ER -