Taste responses in mice lacking taste receptor subunit T1R1

Yoko Kusuhara, Ryusuke Yoshida, Tadahiro Ohkuri, Keiko Nakano, Anja Voigt, Sandra Hübner, Katsumasa Maeda, Ulrich Boehm, Wolfgang Meyerhof, Yuzo Ninomiya

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The T1R1 receptor subunit acts as an umami taste receptor in combination with its partner, T1R3. In addition, metabotropic glutamate receptors (brain and taste variants of mGluR1 and mGluR4) are thought to function as umami taste receptors. To elucidate the function of T1R1 and the contribution of mGluRs to umami taste detection in vivo, we used newly developed knock-out (T1R1-/-) mice, which lack the entire coding region of the Tas1r1 gene and express mCherry in T1R1-expressing cells. Gustatory nerve recordings demonstrated that T1R1-/- mice exhibited a serious deficit in inosine monophosphate-elicited synergy but substantial residual responses to glutamate alone in both chorda tympani and glossopharyngeal nerves. Interestingly, chorda tympani nerve responses to sweeteners were smaller in T1R1-/- mice. Taste cell recordings demonstrated that many mCherry-expressing taste cells in T1R1+/- mice responded to sweet and umami compounds, whereas those in T1R1-/- mice responded to sweet stimuli. The proportion of sweet-responsive cells was smaller in T1R1-/- than in T1R1+/- mice. Single-cell RT-PCR demonstrated that some single mCherry-expressing cells expressed all three T1R subunits. Chorda tympani and glossopharyngeal nerve responses to glutamate were significantly inhibited by addition of mGluR antagonists in both T1R1-/- and T1R1+/- mice. Conditioned taste aversion tests demonstrated that both T1R1-/- and T1R1+/- mice were equally capable of discriminating glutamate from other basic taste stimuli. Avoidance conditioned to glutamate was significantly reduced by addition of mGluR antagonists. These results suggest that T1R1-expressing cells mainly contribute to umami taste synergism and partly to sweet sensitivity and that mGluRs are involved in the detection of umami compounds.

Original languageEnglish
Pages (from-to)1967-1985
Number of pages19
JournalJournal of Physiology
Volume591
Issue number7
DOIs
Publication statusPublished - Apr 1 2013

Fingerprint

Chorda Tympani Nerve
Glutamic Acid
Glossopharyngeal Nerve
Sweetening Agents
Inosine Monophosphate
Metabotropic Glutamate Receptors
Knockout Mice
Polymerase Chain Reaction
Brain
Genes

All Science Journal Classification (ASJC) codes

  • Physiology

Cite this

Kusuhara, Y., Yoshida, R., Ohkuri, T., Nakano, K., Voigt, A., Hübner, S., ... Ninomiya, Y. (2013). Taste responses in mice lacking taste receptor subunit T1R1. Journal of Physiology, 591(7), 1967-1985. https://doi.org/10.1113/jphysiol.2012.236604

Taste responses in mice lacking taste receptor subunit T1R1. / Kusuhara, Yoko; Yoshida, Ryusuke; Ohkuri, Tadahiro; Nakano, Keiko; Voigt, Anja; Hübner, Sandra; Maeda, Katsumasa; Boehm, Ulrich; Meyerhof, Wolfgang; Ninomiya, Yuzo.

In: Journal of Physiology, Vol. 591, No. 7, 01.04.2013, p. 1967-1985.

Research output: Contribution to journalArticle

Kusuhara, Y, Yoshida, R, Ohkuri, T, Nakano, K, Voigt, A, Hübner, S, Maeda, K, Boehm, U, Meyerhof, W & Ninomiya, Y 2013, 'Taste responses in mice lacking taste receptor subunit T1R1', Journal of Physiology, vol. 591, no. 7, pp. 1967-1985. https://doi.org/10.1113/jphysiol.2012.236604
Kusuhara Y, Yoshida R, Ohkuri T, Nakano K, Voigt A, Hübner S et al. Taste responses in mice lacking taste receptor subunit T1R1. Journal of Physiology. 2013 Apr 1;591(7):1967-1985. https://doi.org/10.1113/jphysiol.2012.236604
Kusuhara, Yoko ; Yoshida, Ryusuke ; Ohkuri, Tadahiro ; Nakano, Keiko ; Voigt, Anja ; Hübner, Sandra ; Maeda, Katsumasa ; Boehm, Ulrich ; Meyerhof, Wolfgang ; Ninomiya, Yuzo. / Taste responses in mice lacking taste receptor subunit T1R1. In: Journal of Physiology. 2013 ; Vol. 591, No. 7. pp. 1967-1985.
@article{bee44fce36824efba098d61b1fb0f338,
title = "Taste responses in mice lacking taste receptor subunit T1R1",
abstract = "The T1R1 receptor subunit acts as an umami taste receptor in combination with its partner, T1R3. In addition, metabotropic glutamate receptors (brain and taste variants of mGluR1 and mGluR4) are thought to function as umami taste receptors. To elucidate the function of T1R1 and the contribution of mGluRs to umami taste detection in vivo, we used newly developed knock-out (T1R1-/-) mice, which lack the entire coding region of the Tas1r1 gene and express mCherry in T1R1-expressing cells. Gustatory nerve recordings demonstrated that T1R1-/- mice exhibited a serious deficit in inosine monophosphate-elicited synergy but substantial residual responses to glutamate alone in both chorda tympani and glossopharyngeal nerves. Interestingly, chorda tympani nerve responses to sweeteners were smaller in T1R1-/- mice. Taste cell recordings demonstrated that many mCherry-expressing taste cells in T1R1+/- mice responded to sweet and umami compounds, whereas those in T1R1-/- mice responded to sweet stimuli. The proportion of sweet-responsive cells was smaller in T1R1-/- than in T1R1+/- mice. Single-cell RT-PCR demonstrated that some single mCherry-expressing cells expressed all three T1R subunits. Chorda tympani and glossopharyngeal nerve responses to glutamate were significantly inhibited by addition of mGluR antagonists in both T1R1-/- and T1R1+/- mice. Conditioned taste aversion tests demonstrated that both T1R1-/- and T1R1+/- mice were equally capable of discriminating glutamate from other basic taste stimuli. Avoidance conditioned to glutamate was significantly reduced by addition of mGluR antagonists. These results suggest that T1R1-expressing cells mainly contribute to umami taste synergism and partly to sweet sensitivity and that mGluRs are involved in the detection of umami compounds.",
author = "Yoko Kusuhara and Ryusuke Yoshida and Tadahiro Ohkuri and Keiko Nakano and Anja Voigt and Sandra H{\"u}bner and Katsumasa Maeda and Ulrich Boehm and Wolfgang Meyerhof and Yuzo Ninomiya",
year = "2013",
month = "4",
day = "1",
doi = "10.1113/jphysiol.2012.236604",
language = "English",
volume = "591",
pages = "1967--1985",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Taste responses in mice lacking taste receptor subunit T1R1

AU - Kusuhara, Yoko

AU - Yoshida, Ryusuke

AU - Ohkuri, Tadahiro

AU - Nakano, Keiko

AU - Voigt, Anja

AU - Hübner, Sandra

AU - Maeda, Katsumasa

AU - Boehm, Ulrich

AU - Meyerhof, Wolfgang

AU - Ninomiya, Yuzo

PY - 2013/4/1

Y1 - 2013/4/1

N2 - The T1R1 receptor subunit acts as an umami taste receptor in combination with its partner, T1R3. In addition, metabotropic glutamate receptors (brain and taste variants of mGluR1 and mGluR4) are thought to function as umami taste receptors. To elucidate the function of T1R1 and the contribution of mGluRs to umami taste detection in vivo, we used newly developed knock-out (T1R1-/-) mice, which lack the entire coding region of the Tas1r1 gene and express mCherry in T1R1-expressing cells. Gustatory nerve recordings demonstrated that T1R1-/- mice exhibited a serious deficit in inosine monophosphate-elicited synergy but substantial residual responses to glutamate alone in both chorda tympani and glossopharyngeal nerves. Interestingly, chorda tympani nerve responses to sweeteners were smaller in T1R1-/- mice. Taste cell recordings demonstrated that many mCherry-expressing taste cells in T1R1+/- mice responded to sweet and umami compounds, whereas those in T1R1-/- mice responded to sweet stimuli. The proportion of sweet-responsive cells was smaller in T1R1-/- than in T1R1+/- mice. Single-cell RT-PCR demonstrated that some single mCherry-expressing cells expressed all three T1R subunits. Chorda tympani and glossopharyngeal nerve responses to glutamate were significantly inhibited by addition of mGluR antagonists in both T1R1-/- and T1R1+/- mice. Conditioned taste aversion tests demonstrated that both T1R1-/- and T1R1+/- mice were equally capable of discriminating glutamate from other basic taste stimuli. Avoidance conditioned to glutamate was significantly reduced by addition of mGluR antagonists. These results suggest that T1R1-expressing cells mainly contribute to umami taste synergism and partly to sweet sensitivity and that mGluRs are involved in the detection of umami compounds.

AB - The T1R1 receptor subunit acts as an umami taste receptor in combination with its partner, T1R3. In addition, metabotropic glutamate receptors (brain and taste variants of mGluR1 and mGluR4) are thought to function as umami taste receptors. To elucidate the function of T1R1 and the contribution of mGluRs to umami taste detection in vivo, we used newly developed knock-out (T1R1-/-) mice, which lack the entire coding region of the Tas1r1 gene and express mCherry in T1R1-expressing cells. Gustatory nerve recordings demonstrated that T1R1-/- mice exhibited a serious deficit in inosine monophosphate-elicited synergy but substantial residual responses to glutamate alone in both chorda tympani and glossopharyngeal nerves. Interestingly, chorda tympani nerve responses to sweeteners were smaller in T1R1-/- mice. Taste cell recordings demonstrated that many mCherry-expressing taste cells in T1R1+/- mice responded to sweet and umami compounds, whereas those in T1R1-/- mice responded to sweet stimuli. The proportion of sweet-responsive cells was smaller in T1R1-/- than in T1R1+/- mice. Single-cell RT-PCR demonstrated that some single mCherry-expressing cells expressed all three T1R subunits. Chorda tympani and glossopharyngeal nerve responses to glutamate were significantly inhibited by addition of mGluR antagonists in both T1R1-/- and T1R1+/- mice. Conditioned taste aversion tests demonstrated that both T1R1-/- and T1R1+/- mice were equally capable of discriminating glutamate from other basic taste stimuli. Avoidance conditioned to glutamate was significantly reduced by addition of mGluR antagonists. These results suggest that T1R1-expressing cells mainly contribute to umami taste synergism and partly to sweet sensitivity and that mGluRs are involved in the detection of umami compounds.

UR - http://www.scopus.com/inward/record.url?scp=84875648218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875648218&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2012.236604

DO - 10.1113/jphysiol.2012.236604

M3 - Article

C2 - 23339178

AN - SCOPUS:84875648218

VL - 591

SP - 1967

EP - 1985

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 7

ER -