Taxonomic re-examination of several Japanese Stemphylium strains based on morphological and molecular phylogenetic analyses

Daisuke Kurose, Tomoo Misawa, Takahito Suzui, Kazunori Ichikawa, Gan Kisaki, Long H. Hoang, Naruto Furuya, Kenichi Tsuchiya, Seiya Tsushima, Toyozo Sato

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Abstract

Most of the many Stemphylium species on record as plant pathogens in Japan have been identified by morphology. Using molecular phylogenetic analysis of four loci (rDNA-ITS, EF-1α, GPD, and vmaA-vpsA) combined, we re-examined the taxonomy of 31 Stemphylium strains that had been identified morphologically before or after their deposit in the NIAS Genebank, Japan. In the present study, these Japanese strains were separated into three groups (Groups A, C, and E). Sixteen Japanese strains formed a new subgroup (designated Subgroup C2) that contained no type or ex-type strains of existing species. Four of the strains had been identified correctly, whereas the other 27 strains appear to have been misidentified based on our phylogenetic analysis. We examined the conidial morphology of four representative misidentified strains selected from each clade. While the morphological characteristics of a strain isolated from Welsh onion resembles those of the S. vesicarium type, this strain belongs to Subgroup C2 rather than Subgroup C1 and, therefore, could not be identified at the species level. Conversely, three pathogenic strains isolated from aster, asparagus, and Chinese chive were re-identified based on our combined morphological and phylogenetic data. We propose the scientific names of the aster leaf spot pathogen as S. lycopersici, and the asparagus Stemphylium leaf spot and Chinese chive brown leaf blight pathogens as S. herbarum.

Original languageEnglish
Pages (from-to)358-367
Number of pages10
JournalJournal of General Plant Pathology
Volume81
Issue number5
DOIs
Publication statusPublished - Sep 10 2015

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Stemphylium
phylogeny
leaf spot
chives
asparagus spears
Japanese bunching onions
Japan
leaf blight
pathogens
plant pathogens
taxonomy

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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Taxonomic re-examination of several Japanese Stemphylium strains based on morphological and molecular phylogenetic analyses. / Kurose, Daisuke; Misawa, Tomoo; Suzui, Takahito; Ichikawa, Kazunori; Kisaki, Gan; Hoang, Long H.; Furuya, Naruto; Tsuchiya, Kenichi; Tsushima, Seiya; Sato, Toyozo.

In: Journal of General Plant Pathology, Vol. 81, No. 5, 10.09.2015, p. 358-367.

Research output: Contribution to journalArticle

Kurose, D, Misawa, T, Suzui, T, Ichikawa, K, Kisaki, G, Hoang, LH, Furuya, N, Tsuchiya, K, Tsushima, S & Sato, T 2015, 'Taxonomic re-examination of several Japanese Stemphylium strains based on morphological and molecular phylogenetic analyses', Journal of General Plant Pathology, vol. 81, no. 5, pp. 358-367. https://doi.org/10.1007/s10327-015-0607-x
Kurose, Daisuke ; Misawa, Tomoo ; Suzui, Takahito ; Ichikawa, Kazunori ; Kisaki, Gan ; Hoang, Long H. ; Furuya, Naruto ; Tsuchiya, Kenichi ; Tsushima, Seiya ; Sato, Toyozo. / Taxonomic re-examination of several Japanese Stemphylium strains based on morphological and molecular phylogenetic analyses. In: Journal of General Plant Pathology. 2015 ; Vol. 81, No. 5. pp. 358-367.
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