TCDD disrupts posterior palatogenesis and causes cleft palate

Tomohiro Yamada, Azumi Hirata, Eri Sasabe, Tomohide Yoshimura, Seiji Ohno, Naoya Kitamura, Tetsuya Yamamoto

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18 Citations (Scopus)

Abstract

Dioxins (e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin; TCDD) cause cleft palate at a high rate. A post-fusional split may contribute to the pathogenesis, and tissue fragility may be a concern. The objective of this study was to investigate the effects of TCDD on the palatal epithelium, bone and muscle, which contribute to tissue integrity. ICR mice (10-12 weeks old) were used. TCDD was administered on E12.5 at 40 mg/kg. Immunohistochemical staining for AhR, ER-α, laminin, collagen IV, osteopontin, Runx2, MyoD, and desmin were performed. Furthermore, western blot analysis for osteopontin, Runx2, MyoD, and desmin were performed to evaluate protein expression in the palatal tissue. Immunohistologically, there was little difference in the collagen IV and laminin localization in the palatal epithelium between control versus TCDD-treated mice. Runx2 and osteopontin immunoreactivity decreased in the TCDD-treated palatal bone, and MyoD and desmin decreased in the TCDD-treated palatal muscle. AhR and ER-α immunoreactivity were localized to the normal palatal bone, but ER-α was diminished in the TCDD-treated palate. On western blot analysis, Runx2, MyoD, and desmin were all downregulated in the TCDD-treated palate. TCDD may suppress palatal osteogenesis and myogenesis via AhR, and cause cleft palates via a post-fusional split mechanism, in addition to a failure of palatal fusion.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Cranio-Maxillofacial Surgery
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

Yamada, T., Hirata, A., Sasabe, E., Yoshimura, T., Ohno, S., Kitamura, N., & Yamamoto, T. (2014). TCDD disrupts posterior palatogenesis and causes cleft palate. Journal of Cranio-Maxillofacial Surgery, 42(1), 1-6. https://doi.org/10.1016/j.jcms.2013.01.024