TectoRNP: Self-assembling RNAs with peptide recognition motifs as templates for chemical peptide ligation

Kohei Yamashita, Takahiro Tanaka, Hiroyuki Furuta, Yoshiya Ikawa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

TectoRNA, an artificial RNA with self-assembling ability, has been employed as a structural platform for RNA nanotechnology and RNA synthetic biology. In this study, tectoRNA was applied as a specific template for chemical peptide ligation. On the basis of a self-assembling tectoRNA, we designed and constructed a template RNA that facilitates peptide ligation depending on controlled dimer formation. Two RNA-binding peptides were recognized by two peptide-binding RNA motifs embedded in the template RNA, and chemical ligation was promoted because of the entropic effect of Mg2+-dependent dimerization. In a series of biochemical analyses, we determined the relationship between the structures of the tectoRNA-based templates and the extent of acceleration in peptide ligation.

Original languageEnglish
Pages (from-to)635-642
Number of pages8
JournalJournal of Peptide Science
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 2012

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

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