Telomerase activation and cell proliferation during 7,12- dimethylbenz[a]anthracene-induced hamster cheek pouch carcinogenesis

Tomoki Sumida, Hiroyuki Hamakawa, Kenichi Sogawa, Yang Bao, Hirofumi Zen, Atsuro Sugita, Kenji Nezu, Yasuhito Abe, Hiroaki Tanioka, Norifumi Ueda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Telomerase is a ribonucleoprotein complex intimately involved in cell immortalization and carcinogenesis. This enzyme is activated and stabilizes telomere length in almost all types of cancer. Telomerase may be necessary for continuous cell proliferation. In this study, we analyzed telomerase activity in hamster experimental oral lesions (starting from epithelial hyperplasia through dysplasia, carcinoma in situ, and invasive carcinoma) evoked by 7,12-dimethylbenz[a]anthracene, and in normal mucosa. We also analyzed proliferative activity in these lesions by using immunohistochemical analysis and flow cytometry. Histologically normal epithelium expressed weak telomerase activity. The telomerase activity count increased rapidly in the early stage of carcinogenesis and gradually in the late stage. Cell- proliferative activity closely correlated with progression of disease. These findings indicate that telomerase activation is an early event and that increases in telomerase activity upregulate cell proliferation in chemically induced hamster oral carcinogenesis.

Original languageEnglish
Pages (from-to)164-168
Number of pages5
JournalMolecular Carcinogenesis
Volume25
Issue number3
DOIs
Publication statusPublished - Jul 19 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cancer Research

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