Telomerase activity and telomere length distribution in vascular endothelial cells in a short-term culture under the presence of hydrogen peroxide

Toyoki Maeda, Jing Zhi Guan, Masamichi Koyanagi, Naoki Makino

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Aim: The aim of this study was to assess the biological effects of oxidative stress on human vascular endothelial cells. Methods: The telomeric changes and the alterations of the expression of telomere-associated proteins in human umbilical venous endothelial cells (HUVEC) cultured in the presence of hydrogen peroxide (H2O2) were analyzed. Results: During the culture, the cell growth rate decreased, whereas the telomerase activity of the surviving cells increased. As the H2O2 level increased, long telomeres decreased proportionally, thus resulting in a telomere length distribution that was rich in short telomeres. These observations suggested that H2O2-affected endothelial cells bear telomeric features similar to those of aged cells. In contrast, the expression of telomere-associated proteins, TRF1 and TRF2, showed different changes. TRF1 increased in relation to H2O2 concentration, whereas TRF2 showed no significant change. The surviving cells exposed to H2O2 showed a H2O2-dose dependent increase in telomerase activity, whereas the telomere protein and RNA components were only elevated in low concentrations of H2O2. Conclusions: The increase in telomerase activity and TRF1 protein expression of vascular endothelial cell might show an aspect of cellular protective reaction against oxygen stress. Geriatr Gerontol Int 2013; 13: 774-782.

Original languageEnglish
Pages (from-to)774-782
Number of pages9
JournalGeriatrics and Gerontology International
Volume13
Issue number3
DOIs
Publication statusPublished - Jul 2013

All Science Journal Classification (ASJC) codes

  • Health(social science)
  • Gerontology
  • Geriatrics and Gerontology

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