The effect of the activated slip systems on the temperature dependence of yield stress was investigated in α-Ti by using crystal plasticity finite element method. A model for finite element analysis (FEA) was constructed based on experimental results. The displacement in FEA was applied up to the nominal strain of 4% which is the same strain as the experimental one. Stress-strain curves were obtained, which corresponds to experimental data taken every 50 K between 73 K and 673 K. The used material constants which are temperature dependent were elastic constants, and lattice friction stresses. The lattice friction stresses of basal slip systems were set to be higher than that of pyramidal slip systems at 73 K. Then, the lattice friction stresses were set to be closer as the temperature increases. It was found that the activation of slip systems is strong temperature dependent, and that the yield stress depends on the number of active slip systems.