Temperature-dependent translational regulation of the ER ω-3 fatty acid desaturase gene in wheat root tips

Gorou Horiguchi, Takuichi Fuse, Naoto Kawakami, Hiroaki Kodama, Koh Iba

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The proportion of α-linolenic acid (18:3) among the total fatty acids in root tissue increases as the growth temperature decreases. Endoplasmic reticulum ω-3 fatty acid desaturase is responsible for the production of most of the 18:3 in root tissue. In this study, the effect of temperature on expression of the TaFAD3 gene, which encodes endoplasmic reticulum ω-3 desaturase in wheat (Triticum aestivum L. cv. Chihoku), was analysed at the mRNA and protein levels. In wheat root tips grown at 30 and 10°C, the proportions of 18:3 among total fatty acids were 22% and 55%, respectively. The level of TaFAD3 protein in microsomal preparations of the root tips grown at 10°C was approximately 7.5 times higher than that of the root tips grown at 30°C. The increased level of TaFAD3 protein at the lower temperature was accompanied by enhanced association of TaFAD3 mRNA with polyribosomes. In contrast, the level of TaFAD3 mRNA in root tips grown at 10°C was slightly higher than in those grown at 30°C. These results suggest that, in root tips, the increase in the 18:3 level at low temperature is achieved directly by an increase in the amount of TaFAD3 protein, and that temperature-dependent translational regulation of the TaFAD3 gene, rather than its transcriptional regulation, contributes to modulation of the TaFAD3 protein accumulation.

Original languageEnglish
Pages (from-to)805-813
Number of pages9
JournalPlant Journal
Volume24
Issue number6
DOIs
Publication statusPublished - Dec 1 2000

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'Temperature-dependent translational regulation of the ER ω-3 fatty acid desaturase gene in wheat root tips'. Together they form a unique fingerprint.

  • Cite this