Temperature-modulated adsorption of poly(N-isopropylacrylamide)-grafted ferritin on solid substrate

Yoshikazu Kumashiro, Yasuhiro Ikezoe, Tomohiro Hayashi, Youichi Okabayashi, Kaoru Tamada, Masayuki Yamato, Teruo Okano, Masahiko Hara

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3 Citations (Scopus)

Abstract

Ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) was synthesized by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials. The hydrodynamic diameter of PIPAAm-ferritins in aqueous solution increased at 37°C at a higher protein concentration (>0.2. mg/mL) because of the intermolecular aggregation through the hydrophobic interaction of PIPAAm chains. On the other hand, PIPAAm-ferritins at a lower concentration (<0.2. mg/mL) were unable to increase their size even at 37°C. The adsorption kinetics of PIPAAm-ferritins on hydrophobically modified Si substrate were evaluated with a quartz crystal microbalance in 10. mmol/L Bis-Tris/HCl buffer (pH 5.8) with and without poly(oxyethylene) sorbitan monolaurate (TWEEN 20) (0.05. wt%) as a surfactant. Although the adsorption of PIPAAm-ferritins on hydrophobic Si substrate at 25°C in the buffer with TWEEN 20 was hardly observed, PIPAAm-ferritins were considerably adsorbed on the substrate at 37°C, indicating that the hydrophobic interaction between the substrate and PIPAAm grafts on the ferritins after the destruction of the hydrophobic interaction between the protein and the substrate by TWEEN 20.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume95
DOIs
Publication statusPublished - Jun 15 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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