Iceplant is a halophyte with two different photosynthetic modes of Ca and crassulacean acid metabolism (CAM). Environmental stress, such as a high salinity condition, is known as a trigger for shifting the photosynthetic mode from C3 to CAM in a plant. The application of 6-benzylaminopurine to cell culture medium was effective to induce green calli from leaves of C3 and CAM plants. The photosynthesis of greened calli obtained from C3 and CAM plant leaves showed low temperature adaptation and wide temperature adaptation, respectively. In the calli derived from the C3 plant, the activity of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was higher than that of phosphoenolpyruvate carboxylase (PEPCase), while in the calli from the CAM plant, the activity of PEPCase was higher than that of RuBPCase. Thus, the greened calli showed the original levels of enzymatic activity in their donor plants. The activity of PEPCase in the greened calli obtained from the C3 plant increased with the application of NaCl to the cell culture medium but this effect was not detected on nongreened calli.
All Science Journal Classification (ASJC) codes
- Food Science
- Agronomy and Crop Science