Temperature response of photosynthesis and activities of phosphoenolpyruvate carboxylase and ribulose 1,5-bisphosphate carboxylase in greened calli induced from iceplant, Mesembryanthemum crystallinum L., leaves

Ken Ishimaru, Kazuyuki Saitou, Fumitake Kubota, Waichi Agata

Research output: Contribution to journalArticle

Abstract

Iceplant is a halophyte with two different photosynthetic modes of Ca and crassulacean acid metabolism (CAM). Environmental stress, such as a high salinity condition, is known as a trigger for shifting the photosynthetic mode from C 3 to CAM in a plant. The application of 6-benzylaminopurine to cell culture medium was effective to induce green calli from leaves of C 3 and CAM plants. The photosynthesis of greened calli obtained from C 3 and CAM plant leaves showed low temperature adaptation and wide temperature adaptation, respectively. In the calli derived from the C 3 plant, the activity of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was higher than that of phosphoenolpyruvate carboxylase (PEPCase), while in the calli from the CAM plant, the activity of PEPCase was higher than that of RuBPCase. Thus, the greened calli showed the original levels of enzymatic activity in their donor plants. The activity of PEPCase in the greened calli obtained from the C 3 plant increased with the application of NaCl to the cell culture medium but this effect was not detected on nongreened calli.

Original languageEnglish
Pages (from-to)531-537
Number of pages7
JournalJapanese Journal of Crop Science
Volume66
Issue number4
DOIs
Publication statusPublished - Jan 1 1997

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Mesembryanthemum
Phosphoenolpyruvate Carboxylase
Mesembryanthemum crystallinum
phosphoenolpyruvate carboxylase
Bony Callus
Photosynthesis
ribulose 1,5-diphosphate
Crassulacean acid metabolism
callus
photosynthesis
Temperature
leaves
Acids
temperature
N-benzyladenine
Culture Media
cell culture
Cell Culture Techniques
culture media
Plant Leaves

All Science Journal Classification (ASJC) codes

  • Food Science
  • Agronomy and Crop Science
  • Genetics

Cite this

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title = "Temperature response of photosynthesis and activities of phosphoenolpyruvate carboxylase and ribulose 1,5-bisphosphate carboxylase in greened calli induced from iceplant, Mesembryanthemum crystallinum L., leaves",
abstract = "Iceplant is a halophyte with two different photosynthetic modes of Ca and crassulacean acid metabolism (CAM). Environmental stress, such as a high salinity condition, is known as a trigger for shifting the photosynthetic mode from C 3 to CAM in a plant. The application of 6-benzylaminopurine to cell culture medium was effective to induce green calli from leaves of C 3 and CAM plants. The photosynthesis of greened calli obtained from C 3 and CAM plant leaves showed low temperature adaptation and wide temperature adaptation, respectively. In the calli derived from the C 3 plant, the activity of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was higher than that of phosphoenolpyruvate carboxylase (PEPCase), while in the calli from the CAM plant, the activity of PEPCase was higher than that of RuBPCase. Thus, the greened calli showed the original levels of enzymatic activity in their donor plants. The activity of PEPCase in the greened calli obtained from the C 3 plant increased with the application of NaCl to the cell culture medium but this effect was not detected on nongreened calli.",
author = "Ken Ishimaru and Kazuyuki Saitou and Fumitake Kubota and Waichi Agata",
year = "1997",
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doi = "10.1626/jcs.66.531",
language = "English",
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TY - JOUR

T1 - Temperature response of photosynthesis and activities of phosphoenolpyruvate carboxylase and ribulose 1,5-bisphosphate carboxylase in greened calli induced from iceplant, Mesembryanthemum crystallinum L., leaves

AU - Ishimaru, Ken

AU - Saitou, Kazuyuki

AU - Kubota, Fumitake

AU - Agata, Waichi

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Iceplant is a halophyte with two different photosynthetic modes of Ca and crassulacean acid metabolism (CAM). Environmental stress, such as a high salinity condition, is known as a trigger for shifting the photosynthetic mode from C 3 to CAM in a plant. The application of 6-benzylaminopurine to cell culture medium was effective to induce green calli from leaves of C 3 and CAM plants. The photosynthesis of greened calli obtained from C 3 and CAM plant leaves showed low temperature adaptation and wide temperature adaptation, respectively. In the calli derived from the C 3 plant, the activity of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was higher than that of phosphoenolpyruvate carboxylase (PEPCase), while in the calli from the CAM plant, the activity of PEPCase was higher than that of RuBPCase. Thus, the greened calli showed the original levels of enzymatic activity in their donor plants. The activity of PEPCase in the greened calli obtained from the C 3 plant increased with the application of NaCl to the cell culture medium but this effect was not detected on nongreened calli.

AB - Iceplant is a halophyte with two different photosynthetic modes of Ca and crassulacean acid metabolism (CAM). Environmental stress, such as a high salinity condition, is known as a trigger for shifting the photosynthetic mode from C 3 to CAM in a plant. The application of 6-benzylaminopurine to cell culture medium was effective to induce green calli from leaves of C 3 and CAM plants. The photosynthesis of greened calli obtained from C 3 and CAM plant leaves showed low temperature adaptation and wide temperature adaptation, respectively. In the calli derived from the C 3 plant, the activity of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was higher than that of phosphoenolpyruvate carboxylase (PEPCase), while in the calli from the CAM plant, the activity of PEPCase was higher than that of RuBPCase. Thus, the greened calli showed the original levels of enzymatic activity in their donor plants. The activity of PEPCase in the greened calli obtained from the C 3 plant increased with the application of NaCl to the cell culture medium but this effect was not detected on nongreened calli.

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