The temperature-sensitive mutant of Arabidopsis, chs5, developed chlorotic leaves at restrictive temperatures (15 °C), but almost normal green leaves at permissive temperatures (22 °C). At the restrictive temperature, the chs5 mutation blocked the accumulation of chlorophylls and carotenoids. A temperature-shift analysis revealed that the manifestation of the chlorotic phenotype occurred in young leaf tissues, but did not in mature leaf tissues. Genetic and sequence analysis demonstrated that the chs5 mutation was caused by a single-base change in the coding region of a recently identified CLA1 gene. The CLA1 gene exhibited a high sequence similarity to the genes encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS) localized to the non-mevalonate pathway, which was recently discovered in bacteria and higher plants. In addition, the application of 1-deoxy-D-xylulose, the free sugar of 1-deoxy-D-xylulose 5-phosphate, rescues the defect in the chs5 mutant. These results indicated that the chlorotic phenotype of the chs5 mutant was caused by a defect in DXS activity and that DXS functions preferentially at an early stage of leaf cell development. A transiently expressed green fluorescent protein fused with the CLA1 transit peptide was localized within the chloroplasts in the green cultured cells of tobacco, which suggests that the putative localization of the non-mevalonate pathway is in plastids.
|Number of pages||6|
|Publication status||Published - Jan 2000|
All Science Journal Classification (ASJC) codes
- Plant Science
- Cell Biology