TEMPOL increases NAD+ and improves redox imbalance in obese mice

Mayumi Yamato, Kimika Kawano, Yuki Yamanaka, Misako Saiga, Ken ichi Yamada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Continuous energy conversion is controlled by reduction-oxidation (redox) processes. NAD+ and NADH represent an important redox couple in energy metabolism. 4-Hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL) is a redox-cycling nitroxide that promotes the scavenging of several reactive oxygen species (ROS) and is reduced to hydroxylamine by NADH. TEMPOL is also involved in NAD+ production in the ascorbic acid-glutathione redox cycle. We utilized the chemical properties of TEMPOL to investigate the effects of antioxidants and NAD+/NADH modulators on the metabolic imbalance in obese mice. Increases in the NAD+/NADH ratio by TEMPOL ameliorated the metabolic imbalance when combined with a dietary intervention, changing from a high-fat diet to a normal diet. Plasma levels of the superoxide marker dihydroethidium were higher in mice receiving the dietary intervention compared with a control diet, but were normalized with TEMPOL consumption. These findings provide novel insights into redox regulation in obesity.

Original languageEnglish
Pages (from-to)316-322
Number of pages7
JournalRedox Biology
Volume8
DOIs
Publication statusPublished - Aug 1 2016

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Clinical Biochemistry

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