TEMPOL increases NAD+ and improves redox imbalance in obese mice

Mayumi Yamato, Kimika Kawano, Yuki Yamanaka, Misako Saiga, Ken-Ichi Yamada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Continuous energy conversion is controlled by reduction-oxidation (redox) processes. NAD+ and NADH represent an important redox couple in energy metabolism. 4-Hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPOL) is a redox-cycling nitroxide that promotes the scavenging of several reactive oxygen species (ROS) and is reduced to hydroxylamine by NADH. TEMPOL is also involved in NAD+ production in the ascorbic acid-glutathione redox cycle. We utilized the chemical properties of TEMPOL to investigate the effects of antioxidants and NAD+/NADH modulators on the metabolic imbalance in obese mice. Increases in the NAD+/NADH ratio by TEMPOL ameliorated the metabolic imbalance when combined with a dietary intervention, changing from a high-fat diet to a normal diet. Plasma levels of the superoxide marker dihydroethidium were higher in mice receiving the dietary intervention compared with a control diet, but were normalized with TEMPOL consumption. These findings provide novel insights into redox regulation in obesity.

Original languageEnglish
Pages (from-to)316-322
Number of pages7
JournalRedox Biology
Volume8
DOIs
Publication statusPublished - Aug 1 2016

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Obese Mice
NAD
Oxidation
Nutrition
Diet
Hydroxylamine
Scavenging
High Fat Diet
Energy conversion
Superoxides
Chemical properties
Energy Metabolism
Modulators
Ascorbic Acid
Glutathione
Reactive Oxygen Species
Obesity
Antioxidants
Fats
Plasmas

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Cite this

TEMPOL increases NAD+ and improves redox imbalance in obese mice. / Yamato, Mayumi; Kawano, Kimika; Yamanaka, Yuki; Saiga, Misako; Yamada, Ken-Ichi.

In: Redox Biology, Vol. 8, 01.08.2016, p. 316-322.

Research output: Contribution to journalArticle

Yamato, Mayumi ; Kawano, Kimika ; Yamanaka, Yuki ; Saiga, Misako ; Yamada, Ken-Ichi. / TEMPOL increases NAD+ and improves redox imbalance in obese mice. In: Redox Biology. 2016 ; Vol. 8. pp. 316-322.
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