Temporal and spatial differential expression of chicken germlinespecific proteins cDAZL, CDH and CVH during gametogenesis

Gakushi Kito, Shinya Aramaki, Koji Tanaka, Tomoki Soh, Nobuhiko Yamauchi, Masa Aki Hattori

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Deleted in Azoospermia-Like (DAZL) protein coded by Dazl gene is a germline-specific RNA-binding protein essential for gametogenesis in vertebrates, and the chicken Dazl gene has also been identified in primordial germ cells (PGCs). However, the temporal and spatial expression of chicken DAZL (cDAZL) and its molecular role in germ cell development remain enigmatic. Here, we investigated the subcellular distribution and expression of cDAZL at the various stages by using a polyclonal antibody raised against its C-terminal region and compared them with those of additional germline-specific proteins chicken vasa homologue (CVH) and chicken dead end homologue (CDH). Western blot analysis for cDAZL revealed a single band in the embryonic gonads and premature chicken testis, whereas no band was detected in the premature chicken ovary. Fluorescent immunohistochemistry revealed that cDAZL was present in the nucleus and cytoplasm of circulating PGCs. Cells positive for cDAZL and CVH coexisted in the embryonic gonads and premature chicken testis, in which they were distributed near the basement membrane of seminiferous tubules. Of interest, cDAZL was not found in the premature chicken ovary, whereas CVH and CDH were present in germ cells. Collectively, three germline-specific proteins are expressed in chicken germ cells, but their patterns of expression are temporally and spatially distinct.

Original languageEnglish
Pages (from-to)341-346
Number of pages6
JournalJournal of Reproduction and Development
Volume56
Issue number3
DOIs
Publication statusPublished - Jun 1 2010

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gametogenesis
chickens
germ cells
proteins
gonads
testes
RNA-binding proteins
seminiferous tubules
basement membrane
polyclonal antibodies

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Temporal and spatial differential expression of chicken germlinespecific proteins cDAZL, CDH and CVH during gametogenesis. / Kito, Gakushi; Aramaki, Shinya; Tanaka, Koji; Soh, Tomoki; Yamauchi, Nobuhiko; Hattori, Masa Aki.

In: Journal of Reproduction and Development, Vol. 56, No. 3, 01.06.2010, p. 341-346.

Research output: Contribution to journalArticle

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AU - Yamauchi, Nobuhiko

AU - Hattori, Masa Aki

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