Temporal and spatial expression of TGF-β2 in chicken somites during early embryonic development

Shinya Aramaki, Fuminori Sato, Tomoki Soh, Nobuhiko Yamauchi, Takafumi Sakai, Masa Aki Hattori

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6 Citations (Scopus)

Abstract

A multifunctional growth and differentiation factor TGF-β is expressed at various developmental stages, and its principle role may be involvement in organogenesis. The present study was performed to evaluate the temporal and spatial expression of TGF-β2 mRNA in developing somites of chicken embryos during their early developmental periods. TGF-βs were expressed in various tissues of the whole embryo obtained at stage 26 (5 days of incubation) as revealed by whole-mount in situ hybridization. TGF-β2 mRNA was predominantly expressed in somites as well as the head, branchial arch, wing buds, and leg buds. TGF-β2 mRNA first appeared in the rostral somites on E4, and its expression sites expanded to the middle range of somites at stage 26. At stages 29-31 (6-7 days), expression in the rostral somites disappeared, and it appeared in the caudal somites. TGF-β2 expression was also analyzed in sections of the embryo by in situ hybridization. The expression sites of TGF-β2 were clearly observed in the myotomal somite tips as well as the neural tube. RT-PCR analysis showed that TGF-β2 expression was very low in the blastocyte stage embryo and thereafter increased linearly in the whole trunk until stage 26. These data indicate that TGF-β2 may be a regulatory factor participating in the somitogenesis of chicken embryos.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalJournal of Experimental Zoology Part A: Comparative Experimental Biology
Volume303
Issue number5
DOIs
Publication statusPublished - May 1 2005

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embryo (plant)
embryogenesis
chickens
in situ hybridization
buds
organogenesis
tree trunk
legs
reverse transcriptase polymerase chain reaction
developmental stages

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Temporal and spatial expression of TGF-β2 in chicken somites during early embryonic development. / Aramaki, Shinya; Sato, Fuminori; Soh, Tomoki; Yamauchi, Nobuhiko; Sakai, Takafumi; Hattori, Masa Aki.

In: Journal of Experimental Zoology Part A: Comparative Experimental Biology, Vol. 303, No. 5, 01.05.2005, p. 323-330.

Research output: Contribution to journalArticle

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