Temporal and spatial expression patterns of Cdc25 phosphatase isoforms during early xenopus development

Nobushige Nakajo, Yu Ki Deno, Hiroyuki Ueno, Chihiro Kenmochi, Ken Shimuta, Noriyuki Sagata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In early animal development, cell proliferation and differentiation are tightly linked and coordinated. It is important, therefore, to know how the cell cycle is controlled during early development. Cdc25 phosphatases activate cyclin-dependent kinases (Cdks) and thereby promote cell-cycle progression. In Xenopus laevis, three isoforms of cdc25 have been identified, viz. cdc25A, cdc25B and cdc25C. In this study, we isolated a cDNA encoding a novel Xenopus Cdc25 phosphatase (named cdc25D). We investigated the temporal and spatial expression patterns of the four cdc25 isoforms during early Xenopus development, using RT-PCR and whole-mount in situ hybridization. cdc25A and cdc25C were expressed both maternally and zygotically, whereas cdc25B and cdc25D were expressed zygotically. Both cdc25A and cdc25C were expressed mainly in prospective neural regions, whereas cdc25B was expressed preferentially in the central nervous system (CNS), such as the spinal cord and the brain. Interestingly, cdc25D was expressed in the epidermal ectoderm of the late-neurula embryo, and in the liver diverticulum endoderm of the midtailbud embryo. In agreement with the spatial expression patterns in whole embryos, inhibition of bone morphogenetic protein (BMP), a crucial step for neural induction, induced an upregulation of cdc25B, but a downregulation of cdc25D in animal cap assays. These results indicate that different cdc25 isoforms are differently expressed and play different roles during early Xenopus development.

Original languageEnglish
Pages (from-to)627-632
Number of pages6
JournalInternational Journal of Developmental Biology
Volume55
Issue number6
DOIs
Publication statusPublished - Sep 19 2011

Fingerprint

cdc25 Phosphatases
Xenopus
Protein Isoforms
Embryonic Structures
Cell Cycle
Endoderm
Ectoderm
Bone Morphogenetic Proteins
Cyclin-Dependent Kinases
Diverticulum
Xenopus laevis
In Situ Hybridization
Cell Differentiation
Spinal Cord
Up-Regulation
Down-Regulation
Central Nervous System
Complementary DNA
Cell Proliferation
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Cite this

Temporal and spatial expression patterns of Cdc25 phosphatase isoforms during early xenopus development. / Nakajo, Nobushige; Deno, Yu Ki; Ueno, Hiroyuki; Kenmochi, Chihiro; Shimuta, Ken; Sagata, Noriyuki.

In: International Journal of Developmental Biology, Vol. 55, No. 6, 19.09.2011, p. 627-632.

Research output: Contribution to journalArticle

Nakajo, Nobushige ; Deno, Yu Ki ; Ueno, Hiroyuki ; Kenmochi, Chihiro ; Shimuta, Ken ; Sagata, Noriyuki. / Temporal and spatial expression patterns of Cdc25 phosphatase isoforms during early xenopus development. In: International Journal of Developmental Biology. 2011 ; Vol. 55, No. 6. pp. 627-632.
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