Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats

Jing Xu Zhu, Yasuyuki Sasano, Ichiro Takahashi, Itaru Mizoguchi, Manabu Kagayama

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

It is not known how gene expression of bone extracellular matrix molecules is controlled temporally and spatially, or how it is related with morphological differentiation of osteoblasts during embryonic osteogenesis in vivo. The present study was designed to examine gene expressions of type I collagen, osteonectin, bone sialoprotein, osteopontin, and osteocalcin during mandibular osteogenesis using in situ hybridization. Wistar rat embryos 13-20 days post coitum were used. The condensation of mesenchymal cells was formed in 14-day rat embryonic mandibles and expressed genes of pro-α1(I) collagen, osteonectin, bone sialoprotein and osteopontin. Cuboidal osteoblasts surrounding the uncalcified bone matrix were seen as early as in 15-day embryonic mandibles, while flat osteoblasts lining the surface of the calcified bone were seen from 16-day embryonic mandibles. Cuboidal osteoblasts expressed pro-α1(I) collagen, osteonectin and bone sialoprotein intensely but osteopontin very weakly. In contrast, flat osteoblasts expressed osteopontin very strongly. Osteocytes expressed the extracellular matrix molecules actively, in particular, osteopontin. The present study demonstrated the distinct gene expression pattern of type I collagen, osteonectin, bone sialoprotein, osteopontin and osteocalcin during embryonic mandibular osteogenesis in vivo.

Original languageEnglish
Pages (from-to)25-35
Number of pages11
JournalHistochemical Journal
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

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Bone Matrix
Osteopontin
Osteogenesis
Osteonectin
Integrin-Binding Sialoprotein
Extracellular Matrix
Osteoblasts
Gene Expression
Mandible
Osteocalcin
Collagen Type I
Collagen
Osteocytes
In Situ Hybridization
Wistar Rats
Embryonic Structures
Bone and Bones
Genes

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Cell Biology

Cite this

Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. / Zhu, Jing Xu; Sasano, Yasuyuki; Takahashi, Ichiro; Mizoguchi, Itaru; Kagayama, Manabu.

In: Histochemical Journal, Vol. 33, No. 1, 01.01.2001, p. 25-35.

Research output: Contribution to journalArticle

Zhu, Jing Xu ; Sasano, Yasuyuki ; Takahashi, Ichiro ; Mizoguchi, Itaru ; Kagayama, Manabu. / Temporal and spatial gene expression of major bone extracellular matrix molecules during embryonic mandibular osteogenesis in rats. In: Histochemical Journal. 2001 ; Vol. 33, No. 1. pp. 25-35.
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