Tetrahydrocannabinolic acid synthase, the enzyme controlling marijuana psychoactivity, is secreted into the storage cavity of the glandular trichomes

Supaart Sirikantaramas, Futoshi Taura, Yumi Tanaka, Yu Ishikawa, Satoshi Morimoto, Yukihiro Shoyama

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)

Abstract

Tetrahydrocannabinolic acid (THCA) synthase is the enzyme responsible for the production of tetrahydrocannabinol (THC), the psychoactive component of marijuana (Cannabis sativa L.). We suggest herein that THCA is biosynthesized in the storage cavity of the glandular trichomes based on the following observations. (i) The exclusive expression of THCA synthase was confirmed in the secretory cells of glandular trichomes by reverse transcription-PCR (RT-PCR) analysis. (ii) THCA synthase activity was detected in the storage cavity content. (iii) Transgenic tobacco expressing THCA synthase fused to green fluorescent protein showed fluorescence in the trichome head corresponding to the storage cavity. These results also showed that secretory cells of the glandular trichomes secrete not only metabolites but also biosynthetic enzyme.

Original languageEnglish
Pages (from-to)1578-1582
Number of pages5
JournalPlant and Cell Physiology
Volume46
Issue number9
DOIs
Publication statusPublished - Sep 2005

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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