Tetraploid induction by colchicine treatment and crossing with a diploid reveals less-seeded fruit production in pointed gourd (Trichosanthes dioica roxb.)

Jahidul Hassan, Ikuo Miyajima, Yukio Ozaki, Yuki Mizunoe, Kaori Sakai, Wasimullah Zaland

Research output: Contribution to journalArticle

Abstract

Pointed gourd (Trichosanthes dioica Roxb.) (2n = 2x = 22) is a dioecious cucurbit vegetable and green fruit that is edible after cooking. Consumers prefer to consume seedless or less-seeded fruit because seeds are unpalatable due to their hard coats. Therefore, the cross compatibility between the diploid and induced tetraploid will be helpful for seedless or less-seeded fruit production. Thus, the present study was conducted using mature seeds that were immersed in 0.05%, 0.1%, and 0.5% colchicine for 24, 48, and 72 h to induce tetraploids. These tetraploids were used as parents (male or female) in the inter-ploidy and intra-ploidy crosses. A flow cytometric analysis confirmed the induction of three tetraploids at 0.5% colchicine for 48 and 72 h soaking periods. Among these, two (2) females and one (1) male were differentiated after flower initiation. Crossing between the tetraploid’s maternal and diploid paternal parent (4x × 2x), which were revealed to be compatible, resulted in a similar fruit set rate and shape as those of the diploid. In addition, a seed number of 4x × 2x produced fruits that were drastically reduced to 1.8 seeds per fruit, whereas the natural diploid fruits had 26.4 seeds per fruit. These findings suggest that colchicine-induced tetraploid females are important genetic resources for less-seeded fruit production. The genetic stability of tetraploid clones can easily and effectively be maintained by vine cutting for advanced uses.

Original languageEnglish
Article number370
JournalPlants
Volume9
Issue number3
DOIs
Publication statusPublished - Mar 2020

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

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