Tetratricopeptide repeat (TPR) motifs of p67(phox) participate in interaction with the small GTPase Rac and activation of the phagocyte NADPH oxidase

Hirofumi Koga, Hiroaki Terasawa, Hiroyuki Nunoi, Koichiro Takeshige, Fuyuhiko Inagaki, Hideki Sumimoto

Research output: Contribution to journalArticle

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Abstract

The small GTPase Rac functions as a molecular switch in several important cellular events including cytoskeletal reorganization and activation of the phagocyte NADPH oxidase, the latter of which leads to production of superoxide, a precursor of microbicidal oxidants. During formation of the active oxidase complex at the membrane, the GTP-bound Rac appears to interact with the N-terminal region of p67(phox), another indispensable activator that translocates from the cytosol upon phagocyte stimulation. Here we show that the p67(phox) N terminus lacks the CRIB motif, a well known Rac target, but contains four tetratricopeptide repeat (TPR) motifs with highly α-helical structure. Disruption of any of the N-terminal three TPRs, but the last one, results in defective interaction with Rac, while all the four are required for the NADPH oxidase activation. We also find that Arg-102 in the third repeat is likely involved in binding to Rac via an ionic interaction, and that replacement of this residue with Glu completely abrogates the capability of activating the oxidase both in vivo and in vitro. Thus the TPR motifs of p67(phox) are packed to function as a Rac target, thereby playing a crucial role in the active oxidase complex formation.

Original languageEnglish
Pages (from-to)25051-25060
Number of pages10
JournalJournal of Biological Chemistry
Volume274
Issue number35
DOIs
Publication statusPublished - Aug 27 1999

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Monomeric GTP-Binding Proteins
NADPH Oxidase
Phagocytes
Oxidoreductases
Chemical activation
Guanosine Triphosphate
Oxidants
Superoxides
Cytosol
Switches
Membranes
neutrophil cytosol factor 67K

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tetratricopeptide repeat (TPR) motifs of p67(phox) participate in interaction with the small GTPase Rac and activation of the phagocyte NADPH oxidase. / Koga, Hirofumi; Terasawa, Hiroaki; Nunoi, Hiroyuki; Takeshige, Koichiro; Inagaki, Fuyuhiko; Sumimoto, Hideki.

In: Journal of Biological Chemistry, Vol. 274, No. 35, 27.08.1999, p. 25051-25060.

Research output: Contribution to journalArticle

Koga, Hirofumi ; Terasawa, Hiroaki ; Nunoi, Hiroyuki ; Takeshige, Koichiro ; Inagaki, Fuyuhiko ; Sumimoto, Hideki. / Tetratricopeptide repeat (TPR) motifs of p67(phox) participate in interaction with the small GTPase Rac and activation of the phagocyte NADPH oxidase. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 35. pp. 25051-25060.
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