Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

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Abstract

We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.

Original languageEnglish
Pages (from-to)50-52
Number of pages3
JournalToxicon
Volume125
DOIs
Publication statusPublished - Jan 1 2017

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Takifugu
Saxitoxin
Tetraodontiformes
Tetrodotoxin
Denaturation
Hot Temperature
Ultrafiltration
Amino Acid Sequence
Assays
Amino Acids
tetrodotoxin-binding protein
tributyltin

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

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title = "Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes",
abstract = "We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.",
author = "Hina Satone and Shohei Nonaka and Man Lee and Yohei Shimasaki and Takahiro Kusakabe and Shun-Ichiro Kawabata and Yuji Oshima",
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T1 - Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

AU - Satone, Hina

AU - Nonaka, Shohei

AU - Lee, Man

AU - Shimasaki, Yohei

AU - Kusakabe, Takahiro

AU - Kawabata, Shun-Ichiro

AU - Oshima, Yuji

PY - 2017/1/1

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AB - We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.

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