TEX14 interacts with CEP55 to block cell abscission

Tokuko Iwamori, Naoki Iwamori, Lang Ma, Mark A. Edson, Michael P. Greenbaum, Martin M. Matzuk

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

In somatic cells, abscission, the physical separation of daughter cells at the completion of cytokinesis, requires CEP55, ALIX, and TSG101. In contrast, cytokinesis is arrested prior to abscission in differentiating male germ cells that are interconnected by TEX14-positive intercellular bridges. We have previously shown that targeted deletion of TEX14 disrupts intercellular bridges in all germ cells and causes male sterility. Although these findings demonstrate that intercellular bridges are essential for spermatogenesis, it remains to be shown how TEX14 and other proteins come together to prevent abscission and form stable intercellular bridges. Using a biochemical enrichment of male germ cell intercellular bridges, we identified additional bridge proteins, including CEP55. Although CEP55 is highly expressed in testes at the RNA level, there is no report of the presence of CEP55 in germ cells. We show here that CEP55 becomes a stable component of the intercellular bridge and that an evolutionarily conserved GPPX3Y motif of TEX14 binds strongly to CEP55 to block similar GPPX3Y motifs of ALIX and TSG101 from interacting and localizing to the midbody. Thus, TEX14 prevents the completion of cytokinesis by altering the destiny of CEP55 from a nidus for abscission to an integral component of the intercellular bridge.

Original languageEnglish
Pages (from-to)2280-2292
Number of pages13
JournalMolecular and cellular biology
Volume30
Issue number9
DOIs
Publication statusPublished - May 1 2010

Fingerprint

Germ Cells
Cytokinesis
Cell Separation
Male Infertility
Spermatogenesis
Testis
Proteins
RNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

TEX14 interacts with CEP55 to block cell abscission. / Iwamori, Tokuko; Iwamori, Naoki; Ma, Lang; Edson, Mark A.; Greenbaum, Michael P.; Matzuk, Martin M.

In: Molecular and cellular biology, Vol. 30, No. 9, 01.05.2010, p. 2280-2292.

Research output: Contribution to journalArticle

Iwamori, Tokuko ; Iwamori, Naoki ; Ma, Lang ; Edson, Mark A. ; Greenbaum, Michael P. ; Matzuk, Martin M. / TEX14 interacts with CEP55 to block cell abscission. In: Molecular and cellular biology. 2010 ; Vol. 30, No. 9. pp. 2280-2292.
@article{bebedc0cd16e4ae2a2c03c348fb26df9,
title = "TEX14 interacts with CEP55 to block cell abscission",
abstract = "In somatic cells, abscission, the physical separation of daughter cells at the completion of cytokinesis, requires CEP55, ALIX, and TSG101. In contrast, cytokinesis is arrested prior to abscission in differentiating male germ cells that are interconnected by TEX14-positive intercellular bridges. We have previously shown that targeted deletion of TEX14 disrupts intercellular bridges in all germ cells and causes male sterility. Although these findings demonstrate that intercellular bridges are essential for spermatogenesis, it remains to be shown how TEX14 and other proteins come together to prevent abscission and form stable intercellular bridges. Using a biochemical enrichment of male germ cell intercellular bridges, we identified additional bridge proteins, including CEP55. Although CEP55 is highly expressed in testes at the RNA level, there is no report of the presence of CEP55 in germ cells. We show here that CEP55 becomes a stable component of the intercellular bridge and that an evolutionarily conserved GPPX3Y motif of TEX14 binds strongly to CEP55 to block similar GPPX3Y motifs of ALIX and TSG101 from interacting and localizing to the midbody. Thus, TEX14 prevents the completion of cytokinesis by altering the destiny of CEP55 from a nidus for abscission to an integral component of the intercellular bridge.",
author = "Tokuko Iwamori and Naoki Iwamori and Lang Ma and Edson, {Mark A.} and Greenbaum, {Michael P.} and Matzuk, {Martin M.}",
year = "2010",
month = "5",
day = "1",
doi = "10.1128/MCB.01392-09",
language = "English",
volume = "30",
pages = "2280--2292",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - TEX14 interacts with CEP55 to block cell abscission

AU - Iwamori, Tokuko

AU - Iwamori, Naoki

AU - Ma, Lang

AU - Edson, Mark A.

AU - Greenbaum, Michael P.

AU - Matzuk, Martin M.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - In somatic cells, abscission, the physical separation of daughter cells at the completion of cytokinesis, requires CEP55, ALIX, and TSG101. In contrast, cytokinesis is arrested prior to abscission in differentiating male germ cells that are interconnected by TEX14-positive intercellular bridges. We have previously shown that targeted deletion of TEX14 disrupts intercellular bridges in all germ cells and causes male sterility. Although these findings demonstrate that intercellular bridges are essential for spermatogenesis, it remains to be shown how TEX14 and other proteins come together to prevent abscission and form stable intercellular bridges. Using a biochemical enrichment of male germ cell intercellular bridges, we identified additional bridge proteins, including CEP55. Although CEP55 is highly expressed in testes at the RNA level, there is no report of the presence of CEP55 in germ cells. We show here that CEP55 becomes a stable component of the intercellular bridge and that an evolutionarily conserved GPPX3Y motif of TEX14 binds strongly to CEP55 to block similar GPPX3Y motifs of ALIX and TSG101 from interacting and localizing to the midbody. Thus, TEX14 prevents the completion of cytokinesis by altering the destiny of CEP55 from a nidus for abscission to an integral component of the intercellular bridge.

AB - In somatic cells, abscission, the physical separation of daughter cells at the completion of cytokinesis, requires CEP55, ALIX, and TSG101. In contrast, cytokinesis is arrested prior to abscission in differentiating male germ cells that are interconnected by TEX14-positive intercellular bridges. We have previously shown that targeted deletion of TEX14 disrupts intercellular bridges in all germ cells and causes male sterility. Although these findings demonstrate that intercellular bridges are essential for spermatogenesis, it remains to be shown how TEX14 and other proteins come together to prevent abscission and form stable intercellular bridges. Using a biochemical enrichment of male germ cell intercellular bridges, we identified additional bridge proteins, including CEP55. Although CEP55 is highly expressed in testes at the RNA level, there is no report of the presence of CEP55 in germ cells. We show here that CEP55 becomes a stable component of the intercellular bridge and that an evolutionarily conserved GPPX3Y motif of TEX14 binds strongly to CEP55 to block similar GPPX3Y motifs of ALIX and TSG101 from interacting and localizing to the midbody. Thus, TEX14 prevents the completion of cytokinesis by altering the destiny of CEP55 from a nidus for abscission to an integral component of the intercellular bridge.

UR - http://www.scopus.com/inward/record.url?scp=77950675984&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950675984&partnerID=8YFLogxK

U2 - 10.1128/MCB.01392-09

DO - 10.1128/MCB.01392-09

M3 - Article

VL - 30

SP - 2280

EP - 2292

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 9

ER -