Abstract
Skeletal muscles are formed from two cell lineages, myogenic and fibroblastic. Mesoderm-derived myogenic progenitors form muscle cells whereas fibroblastic cells give rise to the supportive connective tissue of skeletal muscles, such as the tendons and perimysium. It remains unknown how myogenic and fibroblastic cell-cell interactions affect cell fate determination and the organization of skeletal muscle. In the present study, we investigated the functional significance of cell-cell interactions in regulating skeletal muscle development. Our study shows that cranial neural crest (CNC) cells give rise to the fibroblastic cells of the tongue skeletal muscle in mice. Loss of Tgfbr2 in CNC cells (Wnt1-Cre;Tgfbr2flox/flox) results in microglossia with reduced Scleraxis and Fgf10 expression as well as decreased myogenic cell proliferation, reduced cell number and disorganized tongue muscles. Furthermore, TGF-β2 beads induced the expression of Scleraxis in tongue explant cultures. The addition of FGF10 rescued the muscle cell number in Wnt1-Cre;Tgfbr2flox/flox mice. Thus, TGF-β induced FGF10 signaling has a critical function in regulating tissue-tissue interaction during tongue skeletal muscle development.
| Original language | English |
|---|---|
| Pages (from-to) | 186-195 |
| Number of pages | 10 |
| Journal | Developmental Biology |
| Volume | 341 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 2010 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Developmental Biology
- Cell Biology
Cite this
TGF-β mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis. / Hosokawa, Ryoichi; Oka, Kyoko; Yamaza, Takayoshi; Iwata, Junichi; Urata, Mark; Xu, Xun; Bringas, Pablo; Nonaka, Kazuaki; Chai Yang, Y.
In: Developmental Biology, Vol. 341, No. 1, 05.2010, p. 186-195.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - TGF-β mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis
AU - Hosokawa, Ryoichi
AU - Oka, Kyoko
AU - Yamaza, Takayoshi
AU - Iwata, Junichi
AU - Urata, Mark
AU - Xu, Xun
AU - Bringas, Pablo
AU - Nonaka, Kazuaki
AU - Chai Yang, Y.
PY - 2010/5
Y1 - 2010/5
N2 - Skeletal muscles are formed from two cell lineages, myogenic and fibroblastic. Mesoderm-derived myogenic progenitors form muscle cells whereas fibroblastic cells give rise to the supportive connective tissue of skeletal muscles, such as the tendons and perimysium. It remains unknown how myogenic and fibroblastic cell-cell interactions affect cell fate determination and the organization of skeletal muscle. In the present study, we investigated the functional significance of cell-cell interactions in regulating skeletal muscle development. Our study shows that cranial neural crest (CNC) cells give rise to the fibroblastic cells of the tongue skeletal muscle in mice. Loss of Tgfbr2 in CNC cells (Wnt1-Cre;Tgfbr2flox/flox) results in microglossia with reduced Scleraxis and Fgf10 expression as well as decreased myogenic cell proliferation, reduced cell number and disorganized tongue muscles. Furthermore, TGF-β2 beads induced the expression of Scleraxis in tongue explant cultures. The addition of FGF10 rescued the muscle cell number in Wnt1-Cre;Tgfbr2flox/flox mice. Thus, TGF-β induced FGF10 signaling has a critical function in regulating tissue-tissue interaction during tongue skeletal muscle development.
AB - Skeletal muscles are formed from two cell lineages, myogenic and fibroblastic. Mesoderm-derived myogenic progenitors form muscle cells whereas fibroblastic cells give rise to the supportive connective tissue of skeletal muscles, such as the tendons and perimysium. It remains unknown how myogenic and fibroblastic cell-cell interactions affect cell fate determination and the organization of skeletal muscle. In the present study, we investigated the functional significance of cell-cell interactions in regulating skeletal muscle development. Our study shows that cranial neural crest (CNC) cells give rise to the fibroblastic cells of the tongue skeletal muscle in mice. Loss of Tgfbr2 in CNC cells (Wnt1-Cre;Tgfbr2flox/flox) results in microglossia with reduced Scleraxis and Fgf10 expression as well as decreased myogenic cell proliferation, reduced cell number and disorganized tongue muscles. Furthermore, TGF-β2 beads induced the expression of Scleraxis in tongue explant cultures. The addition of FGF10 rescued the muscle cell number in Wnt1-Cre;Tgfbr2flox/flox mice. Thus, TGF-β induced FGF10 signaling has a critical function in regulating tissue-tissue interaction during tongue skeletal muscle development.
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UR - http://www.scopus.com/inward/citedby.url?scp=77951216408&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2010.02.030
DO - 10.1016/j.ydbio.2010.02.030
M3 - Article
C2 - 20193675
AN - SCOPUS:77951216408
VL - 341
SP - 186
EP - 195
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -