TGF-β mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis

Ryoichi Hosokawa, Kyoko Oka, Takayoshi Yamaza, Junichi Iwata, Mark Urata, Xun Xu, Pablo Bringas, Kazuaki Nonaka, Y. Chai Yang

Research output: Contribution to journalArticle

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Abstract

Skeletal muscles are formed from two cell lineages, myogenic and fibroblastic. Mesoderm-derived myogenic progenitors form muscle cells whereas fibroblastic cells give rise to the supportive connective tissue of skeletal muscles, such as the tendons and perimysium. It remains unknown how myogenic and fibroblastic cell-cell interactions affect cell fate determination and the organization of skeletal muscle. In the present study, we investigated the functional significance of cell-cell interactions in regulating skeletal muscle development. Our study shows that cranial neural crest (CNC) cells give rise to the fibroblastic cells of the tongue skeletal muscle in mice. Loss of Tgfbr2 in CNC cells (Wnt1-Cre;Tgfbr2flox/flox) results in microglossia with reduced Scleraxis and Fgf10 expression as well as decreased myogenic cell proliferation, reduced cell number and disorganized tongue muscles. Furthermore, TGF-β2 beads induced the expression of Scleraxis in tongue explant cultures. The addition of FGF10 rescued the muscle cell number in Wnt1-Cre;Tgfbr2flox/flox mice. Thus, TGF-β induced FGF10 signaling has a critical function in regulating tissue-tissue interaction during tongue skeletal muscle development.

Original languageEnglish
Pages (from-to)186-195
Number of pages10
JournalDevelopmental Biology
Volume341
Issue number1
DOIs
Publication statusPublished - May 2010

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Neural Crest
Morphogenesis
Tongue
Skeletal Muscle
Stem Cells
Muscle Development
Cell Communication
Muscle Cells
Tongue Diseases
Cell Count
Cell Lineage
Mesoderm
Tendons
Connective Tissue
Cell Proliferation
Muscles

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

TGF-β mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis. / Hosokawa, Ryoichi; Oka, Kyoko; Yamaza, Takayoshi; Iwata, Junichi; Urata, Mark; Xu, Xun; Bringas, Pablo; Nonaka, Kazuaki; Chai Yang, Y.

In: Developmental Biology, Vol. 341, No. 1, 05.2010, p. 186-195.

Research output: Contribution to journalArticle

Hosokawa, Ryoichi ; Oka, Kyoko ; Yamaza, Takayoshi ; Iwata, Junichi ; Urata, Mark ; Xu, Xun ; Bringas, Pablo ; Nonaka, Kazuaki ; Chai Yang, Y. / TGF-β mediated FGF10 signaling in cranial neural crest cells controls development of myogenic progenitor cells through tissue-tissue interactions during tongue morphogenesis. In: Developmental Biology. 2010 ; Vol. 341, No. 1. pp. 186-195.
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