Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF

Feng Jun Mei, Takaaki Ishizu, Hiroyuki Murai, Manabu Osoegawa, Motozumi Minohara, Kun Nan Zhang, Jun-Ichi Kira

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Abstract

To investigate the intra- and extracellular levels of various cytokines and chemokines in CSF in chronic inflammatory demyelinating polyneuropathy (CIDP) and vasculitic neuropathy (VN), 16 cytokines, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17, IFN-γ, TNF-α, G-CSF, MCP-1 and MIP-1β, were measured in CSF supernatant by a multiplexed fluorescent bead-based immunoassay and intracellular production of IFN-γ and IL-4 in CSF CD4 + T cells were simultaneously measured by flow cytometry in 14 patients with CIDP, 8 patients with VN and 25 patients with other noninflammatory neurologic diseases (OND). In the CSF supernatant, a significant increase of IL-17, IL-8 and IL-6, and a significant decrease of IL-4, IL-5 and IL-7 levels were detected in pretreated CIDP as compared with OND. A significant increase of IL-6, IL-8 and IL-10 levels was found in pretreated VN. Both IL-17 and IL-8 levels correlated strongly with CSF protein levels in CIDP, although the correlation of IL-6 levels was weak. In CSF CD4 + T cells, IFN-γ + IL-4 - cell percentages were markedly elevated in CIDP compared with OND, but not in VN, resulting in a significant increase of intracellular IFN-γ/IL-4 ratio in CIDP, even in the absence of CSF pleocytosis. The nonresponders to intravenous immunoglobulins (IVIGs) showed a significantly lower IFN-γ - IL-4 + CD4 + T cell percentage, and tended to have a higher intracellular IFN-γ/IL-4 ratio than the responders in CSF. Marked upregulation of Th1 cytokine, IL-17, and downregulation of Th2 cytokines, together with infiltration of IFN-γ-producing CD4 + T cells are useful markers for CIDP, while several Th2 cytokines are upregulated in VN in CSF.

Original languageEnglish
Pages (from-to)75-85
Number of pages11
JournalJournal of the Neurological Sciences
Volume228
Issue number1
DOIs
Publication statusPublished - Jan 15 2005

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Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Interleukin-4
Interleukin-17
Interleukin-8
Cytokines
Interleukin-6
Nervous System Diseases
T-Lymphocytes
Interleukin-7
Interleukin-5
Interleukin-10
Macrophage Colony-Stimulating Factor
Interleukin-13
Intravenous Immunoglobulins
Leukocytosis
Granulocyte Colony-Stimulating Factor
Interleukin-12
Interleukin-1
Chemokines
Immunoassay

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF. / Mei, Feng Jun; Ishizu, Takaaki; Murai, Hiroyuki; Osoegawa, Manabu; Minohara, Motozumi; Zhang, Kun Nan; Kira, Jun-Ichi.

In: Journal of the Neurological Sciences, Vol. 228, No. 1, 15.01.2005, p. 75-85.

Research output: Contribution to journalArticle

Mei, FJ, Ishizu, T, Murai, H, Osoegawa, M, Minohara, M, Zhang, KN & Kira, J-I 2005, 'Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF', Journal of the Neurological Sciences, vol. 228, no. 1, pp. 75-85. https://doi.org/10.1016/j.jns.2004.10.001
Mei FJ, Ishizu T, Murai H, Osoegawa M, Minohara M, Zhang KN et al. Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF. Journal of the Neurological Sciences. 2005 Jan 15;228(1):75-85. https://doi.org/10.1016/j.jns.2004.10.001
Mei, Feng Jun ; Ishizu, Takaaki ; Murai, Hiroyuki ; Osoegawa, Manabu ; Minohara, Motozumi ; Zhang, Kun Nan ; Kira, Jun-Ichi. / Th1 shift in CIDP versus Th2 shift in vasculitic neuropathy in CSF. In: Journal of the Neurological Sciences. 2005 ; Vol. 228, No. 1. pp. 75-85.
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