Th2 shift in mononeuritis multiplex and increase of Th2 cells in chronic inflammatory demyelinating polyneuropathy: An intracellular cytokine analysis

Izumi Horiuchi, Hirofumi Ochi, Hiroyuki Murai, Manabu Osoegawa, Motozumi Minohara, Hirokazu Furuya, Jun Ichi Kira

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To elucidate the T helper 1 (Th1)/T helper 2 (Th2) balance in various inflammatory neuropathies, we measured the ratio of intracellular interferon-γ (IFN-γ)-positive to IL-4-positive cells (intracellular IFN-γ/IL-4 ratio) by flow cytometry in peripheral blood CD4+ T cells of 14 patients with mononeuritis multiplex (MNM), 12 patients with chronic inflammatory demyelinating polyneuropathy (CIDP), 10 patients with Guillain-Barré syndrome (GBS), 23 patients with neurodegenerative disorders and 36 healthy controls by intracellular labeling. The patients with MNM showed a significantly lower intracellular IFN-γ/IL-4 ratio (P<0.05) and higher IL-4+/IFN-γ- cell percentages (P<0.05) than the controls. The increase of IL-4+/IFN-γ- cell percentages was especially prominent in MNM of unknown etiology (P<0.005). The patients with CIDP also showed significantly higher IL-4+/IFN-γ- cell percentages (P<0.05) than the controls. The IL-4+/IFN-γ- cell percentages were increased in some patients with GBS, but the difference was not significant compared with the controls. Thus, our results suggest that a Th2 shift is a characteristic of MNM and may play an important role in the development of the disease.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalJournal of the Neurological Sciences
Volume193
Issue number1
DOIs
Publication statusPublished - Dec 15 2001

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Chronic Inflammatory Demyelinating Polyradiculoneuropathy
Mononeuropathies
Th2 Cells
Interleukin-4
Interferons
Cytokines
Neurodegenerative Diseases
Flow Cytometry
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

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Th2 shift in mononeuritis multiplex and increase of Th2 cells in chronic inflammatory demyelinating polyneuropathy : An intracellular cytokine analysis. / Horiuchi, Izumi; Ochi, Hirofumi; Murai, Hiroyuki; Osoegawa, Manabu; Minohara, Motozumi; Furuya, Hirokazu; Kira, Jun Ichi.

In: Journal of the Neurological Sciences, Vol. 193, No. 1, 15.12.2001, p. 49-52.

Research output: Contribution to journalArticle

Horiuchi, Izumi ; Ochi, Hirofumi ; Murai, Hiroyuki ; Osoegawa, Manabu ; Minohara, Motozumi ; Furuya, Hirokazu ; Kira, Jun Ichi. / Th2 shift in mononeuritis multiplex and increase of Th2 cells in chronic inflammatory demyelinating polyneuropathy : An intracellular cytokine analysis. In: Journal of the Neurological Sciences. 2001 ; Vol. 193, No. 1. pp. 49-52.
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