The 21-kDa polypeptide (VAP21) in the rabies virion is a CD99-related host cell protein

Junji Sagara, Tadafumi S. Tochikura, Hajime Tanaka, Yoshihiro Baba, Shoichiro Tsukita, Sachiko Tsukita, Akihiko Kawai

Research output: Contribution to journalArticle

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Abstract

In our monoclonal antibody (MAb) stocks prepared against the BHK-21 cell antigens, two (11875 and 28276) recognized a 21-kDa polypeptide (referred to as VAP21) which is efficiently incorporated into the rabies virion. By using these MAbs, we isolated the cDNA clones that encoded a polypeptide of 144 amino acids from our BHK-21 cell cDNA library. Based on the following evidence, the cDNA was assumed to encode a full-length sequence of VAP21 antigen: i) expression of the eDNA in animal cells resulted in the production of a polypeptide recognized by the two MAbs, and its electrophoretic mobility was the same as that of authentic VAP21 antigen; and ii) immunization with the products from the cDNA-transformed E. coli cells raised specific antibodies in rabbits that recognized a 21-kDa polypeptide in the virion. From the deduced amino acid sequence, it is suggested that the VAP21 antigen has a molecular structure of type-I transmembrane protein containing characteristic proline-rich and glycine-rich regions in its ectodomain. Homology searches resulted in finding homologous sequences (totally about 40 % homology) in the human MIC2 gene product (CD99; 32-kDa) of T lymphocytes. These results suggest that the VAP21 antigen in the rabies virion is a cellular CD99-related transmembrane protein.

Original languageEnglish
Pages (from-to)289-297
Number of pages9
JournalMICROBIOLOGY and IMMUNOLOGY
Volume42
Issue number4
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

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Rabies
Virion
Antigens
Peptides
Complementary DNA
Proteins
Sequence Homology
Molecular Structure
Gene Library
Proline
Glycine
Amino Acid Sequence
Immunization
Clone Cells
Monoclonal Antibodies
Escherichia coli
Rabbits
T-Lymphocytes
Amino Acids
Antibodies

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

Cite this

The 21-kDa polypeptide (VAP21) in the rabies virion is a CD99-related host cell protein. / Sagara, Junji; Tochikura, Tadafumi S.; Tanaka, Hajime; Baba, Yoshihiro; Tsukita, Shoichiro; Tsukita, Sachiko; Kawai, Akihiko.

In: MICROBIOLOGY and IMMUNOLOGY, Vol. 42, No. 4, 01.01.1998, p. 289-297.

Research output: Contribution to journalArticle

Sagara, Junji ; Tochikura, Tadafumi S. ; Tanaka, Hajime ; Baba, Yoshihiro ; Tsukita, Shoichiro ; Tsukita, Sachiko ; Kawai, Akihiko. / The 21-kDa polypeptide (VAP21) in the rabies virion is a CD99-related host cell protein. In: MICROBIOLOGY and IMMUNOLOGY. 1998 ; Vol. 42, No. 4. pp. 289-297.
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