The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain

Bon chu Chung, Meng Chun Hu, Char Chang Lai, Cheng Hui Lin

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.

Original languageEnglish
Pages (from-to)276-281
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number1
DOIs
Publication statusPublished - Apr 14 1989

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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