The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain

Bon Chu Chung, Meng Chun Hu, Char Chang Lai, Cheng Hui Lin

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.

Original languageEnglish
Pages (from-to)276-281
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume160
Issue number1
DOIs
Publication statusPublished - Apr 14 1989

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Genes
DNA
Transcription
Reporter Genes
Assays
Steroids
Tissue

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain. / Chung, Bon Chu; Hu, Meng Chun; Lai, Char Chang; Lin, Cheng Hui.

In: Biochemical and Biophysical Research Communications, Vol. 160, No. 1, 14.04.1989, p. 276-281.

Research output: Contribution to journalArticle

Chung, Bon Chu ; Hu, Meng Chun ; Lai, Char Chang ; Lin, Cheng Hui. / The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 160, No. 1. pp. 276-281.
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