The 67 kDa laminin receptor as a primary determinant of anti-allergic effects of O-methylated EGCG

Yoshinori Fujimura, Daisuke Umeda, Satomi Yano, Mari Maeda-Yamamoto, Koji Yamada, Hirofumi Tachibana

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Previously we have reported that the O-methylated derivative of (-)-epigallocatechin-3-O-gallate (EGCG), (-)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me), possesses anti-allergic activities such as inhibition of histamine release and suppression of the high-affinity IgE receptor (FcεRI) expression. However, the underlying mechanism is still unclear. Recently we have identified the 67 kDa laminin receptor (67LR) as a cell-surface receptor that can mediate biological activities of EGCG. Here we show that the suppression of myosin II regulatory light chain (MRLC) phosphorylation through the cell-surface binding to the 67 LR contributes to the inhibitory effect of EGCG3"Me on the histamine release from the human basophilic KU812 cells. The 67LR also mediated the EGCG3"Me-induced suppression of FcεRI expression by reducing ERK1/2 phosphorylation. These results suggest that anti-allergic effects of EGCG3"Me may be triggered by the inhibition of MRLC or ERK1/2 phosphorylation mediated through the cell-surface 67LR.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume364
Issue number1
DOIs
Publication statusPublished - Dec 7 2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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