The action of microsecond-pulsed plasma-activated media on the inactivation of human lung cancer cells

Naresh Kumar; Ji Hoon Park; Su Nam Jeon; Bong Sang Park; Eun Ha Choi; Pankaj Attri

doi:10.1088/0022-3727/49/11/115401

The action of microsecond-pulsed plasma-activated media on the inactivation of human lung cancer cells

Naresh Kumar, Ji Hoon Park, Su Nam Jeon, Bong Sang Park, Eun Ha Choi, Pankaj Attri

Center of Plasma Nono-interface Engineering

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

In the present work, we have generated reactive species (RS) through microsecond-pulsed plasma (MPP) in the cell culture media using a Marx generator with point-point electrodes of approximately 0.06 J discharge energy/pulse. RS generated in culture media through MPP have a selective action between growth of the H460 lung cancer cells and L132 normal lung cells. We observed that MPP-activated media (MPP-AM) induced apoptosis on H460 lung cancer cells through an oxidative DNA damage cascade. Additionally, we studied the apoptosis-related mRNA expression, DNA oxidation and polymerase-1 (PARP-1) cleaved analysis from treated cancer cells. The result proves that radicals generated through MPP play a pivotal role in the activation of media that induces the selective killing effect.

Original language	English
Article number	115401
Journal	Journal of Physics D: Applied Physics
Volume	49
Issue number	11
DOIs	https://doi.org/10.1088/0022-3727/49/11/115401
Publication status	Published - Feb 17 2016

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Acoustics and Ultrasonics
Surfaces, Coatings and Films

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title = "The action of microsecond-pulsed plasma-activated media on the inactivation of human lung cancer cells",

abstract = "In the present work, we have generated reactive species (RS) through microsecond-pulsed plasma (MPP) in the cell culture media using a Marx generator with point-point electrodes of approximately 0.06 J discharge energy/pulse. RS generated in culture media through MPP have a selective action between growth of the H460 lung cancer cells and L132 normal lung cells. We observed that MPP-activated media (MPP-AM) induced apoptosis on H460 lung cancer cells through an oxidative DNA damage cascade. Additionally, we studied the apoptosis-related mRNA expression, DNA oxidation and polymerase-1 (PARP-1) cleaved analysis from treated cancer cells. The result proves that radicals generated through MPP play a pivotal role in the activation of media that induces the selective killing effect.",

author = "Naresh Kumar and Park, {Ji Hoon} and Jeon, {Su Nam} and Park, {Bong Sang} and Choi, {Eun Ha} and Pankaj Attri",

note = "Funding Information: We gratefully acknowledge the grant provided by the SRC program of the National Research Foundation of Korea (NRF), funded by the Korean Government (MEST) (No. 20100029418) and in part by Kwangwoon University 2015. PA is thankful to FY 2015 Japan Society for the Promotion of Science (JSPS) invitation fellowship.",

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AU - Choi, Eun Ha

AU - Attri, Pankaj

N1 - Funding Information: We gratefully acknowledge the grant provided by the SRC program of the National Research Foundation of Korea (NRF), funded by the Korean Government (MEST) (No. 20100029418) and in part by Kwangwoon University 2015. PA is thankful to FY 2015 Japan Society for the Promotion of Science (JSPS) invitation fellowship.

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Y1 - 2016/2/17

N2 - In the present work, we have generated reactive species (RS) through microsecond-pulsed plasma (MPP) in the cell culture media using a Marx generator with point-point electrodes of approximately 0.06 J discharge energy/pulse. RS generated in culture media through MPP have a selective action between growth of the H460 lung cancer cells and L132 normal lung cells. We observed that MPP-activated media (MPP-AM) induced apoptosis on H460 lung cancer cells through an oxidative DNA damage cascade. Additionally, we studied the apoptosis-related mRNA expression, DNA oxidation and polymerase-1 (PARP-1) cleaved analysis from treated cancer cells. The result proves that radicals generated through MPP play a pivotal role in the activation of media that induces the selective killing effect.

AB - In the present work, we have generated reactive species (RS) through microsecond-pulsed plasma (MPP) in the cell culture media using a Marx generator with point-point electrodes of approximately 0.06 J discharge energy/pulse. RS generated in culture media through MPP have a selective action between growth of the H460 lung cancer cells and L132 normal lung cells. We observed that MPP-activated media (MPP-AM) induced apoptosis on H460 lung cancer cells through an oxidative DNA damage cascade. Additionally, we studied the apoptosis-related mRNA expression, DNA oxidation and polymerase-1 (PARP-1) cleaved analysis from treated cancer cells. The result proves that radicals generated through MPP play a pivotal role in the activation of media that induces the selective killing effect.

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The action of microsecond-pulsed plasma-activated media on the inactivation of human lung cancer cells

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